大肠杆菌中的转化：感受态细胞的低温保存。
Transformation in Escherichia coli: cryogenic preservation of competent cells.
作者信息
Morrison D A
出版信息
J Bacteriol. 1977 Oct;132(1):349-51. doi: 10.1128/jb.132.1.349-351.1977.
Abstract
Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.
摘要
用冷的0.1M氯化钙处理以制备用于转化的大肠杆菌细胞，在-82℃下于15%甘油中储存后仍保持存活且具有感受态；解冻后的细胞样品每微克质粒脱氧核糖核酸可产生多达10⁶个转化体。
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