Chen Jie, Gong Shiyu, Wan Xuzhi, Gao Xiaoxiang, Wang Change, Zeng Feng, Zhao Chao, Liu Bin, Huang Ying
College of Food Science Fujian Agriculture and Forestry University Fuzhou China.
National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University Fuzhou China.
Food Sci Nutr. 2020 Dec 11;9(1):459-468. doi: 10.1002/fsn3.2014. eCollection 2021 Jan.
The aim of this study was to explore the effects and mechanisms of 95% ethanol extract of (CPE95) on lipid metabolism in hyperlipidemic rats. For the sake of chemical composition analysis of CPE95, liquid chromatography-mass spectrometry (LC-MS) was used for determination. After treatment with CPE95, serum high-density lipoprotein cholesterol content of the hyperlipidemic rats was increased, while the contents of cholesterol, triglyceride, and low-density lipoprotein cholesterol were decreased strikingly. Moreover, the result of histopathology analysis showed that the accumulation and fatty deformation of the livers were relieved. Real-time quantitative PCR and Western blotting were used to determine the expression levels of lipid metabolism-related genes. The gene expression level of adenosine 5'-monophosphate-activated protein kinase was descended, and expressions of sterol regulatory element-binding protein-1c, acetyl-CoA carboxylase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase were all downregulated in the CPE95-treated rats. It suggested that CPE95 may effectively improve the hyperlipidemia in rats and would be potential for functional food component to reduce blood lipid.
本研究旨在探讨[具体植物名称]95%乙醇提取物（CPE95）对高脂血症大鼠脂质代谢的影响及其机制。为了对CPE95进行化学成分分析，采用液相色谱-质谱联用（LC-MS）进行测定。用CPE95处理后，高脂血症大鼠血清高密度脂蛋白胆固醇含量升高，而胆固醇、甘油三酯和低密度脂蛋白胆固醇含量显著降低。此外，组织病理学分析结果显示肝脏的脂质蓄积和脂肪变性得到缓解。采用实时定量PCR和蛋白质免疫印迹法测定脂质代谢相关基因的表达水平。在CPE95处理的大鼠中，5'-单磷酸腺苷激活蛋白激酶的基因表达水平下降，固醇调节元件结合蛋白-1c、乙酰辅酶A羧化酶和3-羟基-3-甲基戊二酰辅酶A还原酶的表达均下调。这表明CPE95可能有效改善大鼠高脂血症，有望成为具有降血脂作用的功能性食品成分。
