College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, China.
Int J Mol Sci. 2018 Oct 9;19(10):3075. doi: 10.3390/ijms19103075.
Ultra-high performance liquid chromatography coupled with photo-diode array detector and electrospray ionization-mass spectrometry was employed to analyze the major fatty acids in 95% ethanol extract (SPL95). The effects of SPL95 on hepatoprotection were evaluated, including liver tissue histopathology, liver, and serum biochemical analysis. The active principle of SPL95 revealed a hypolipidemic effect, as indicated by down-regulating the mRNA and protein levels of sterol regulatory element-binding transcription factor-1c, 3-hydroxy-3-methyl glutaryl coenzyme A reductase, acetyl CoA carboxylase pathway, and upregulating adenosine 5'-monophosphate-activated protein kinase-α in liver. SPL95 enriched the beneficial bacteria, including , , , , and . Treatment with SPL95 led to a decrease in microbes, such as , , , , and , which were positively correlated with serum triglyceride, total cholesterol, and low-density-lipoprotein cholesterol levels, but negatively correlated with the serum high-density-lipoprotein cholesterol levels. These results provide evidence that the fatty acid from SPL95 may be used as a novel adjuvant therapy and functional food to regulate gut microbiota in obese and diabetic individuals.
采用超高效液相色谱-光电二极管阵列检测器-电喷雾电离-质谱联用技术分析 95%乙醇提取物（SPL95）中的主要脂肪酸。评价了 SPL95 的保肝作用，包括肝组织病理、肝和血清生化分析。SPL95 的活性成分显示出降血脂作用，表现为下调肝组织固醇调节元件结合转录因子-1c、3-羟-3-甲基戊二酰辅酶 A 还原酶、乙酰辅酶 A 羧化酶途径的 mRNA 和蛋白水平，上调肝中腺苷 5'-单磷酸激活蛋白激酶-α。SPL95 富集了有益菌，包括、、、、和。用 SPL95 处理后，与血清甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平呈正相关，但与血清高密度脂蛋白胆固醇水平呈负相关的微生物，如、、、和，其数量减少。这些结果为 SPL95 的脂肪酸可作为肥胖和糖尿病患者调节肠道微生物群的新型辅助治疗和功能性食品提供了证据。
