Zhang Yurong, Guo Yuan-Ming, Li Tie-Jun, Chen Ching-Hung, Shen Kang-Ning, Hsiao Chung-Der
Marine Fisheries Research Institute of Zhejiang Province, Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan, Zhejiang, China.
WeThink Biotech Inc., Taoyuan, Taiwan.
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):2-3. doi: 10.1080/23802359.2015.1137792.
The complete chloroplast DNA (cpDNA) of a famous red alga of the family Gracilariaceae, , was deduced by using next-generation sequencing and assembly technology. The complete cpDNA of consists of 182 505 bp and encodes 230 unique genes consisting 204 protein-coding genes (PCGs), 21 transfer RNA genes, 3 ribosomal RNA genes, 1 transfer-messenger RNA genes and 1 non-coding RNA genes. Among 204 PCGs, ccsA gene is interrupted by a intron. Unlike the typical quadripartite structure (a pair of inverted repeats separated by the small single-copy and large single-copy units) of cpDNA in higher plants, the complete cpDNA of is very compact, containing no inverted repeat and just one copy of rRNA gene cluster consisting of 16S, 23S and 5S rRNA genes. The genic regions account for 83.7% of whole cpDNA genome, and the G + C content of the cpDNA was 27.4%. The low G + C content of cpDNA is largely contributed by high A + T content in the PCGs and non-coding regions. A phylogenetic analysis of the 15 complete cpDNA from rhodophyta shows that is closely related to macroalga . The complete cpDNA of provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for rhodophyta.
利用新一代测序和组装技术推导了江蓠科一种著名红藻的完整叶绿体DNA（cpDNA）。该红藻的完整cpDNA由182505个碱基对组成，编码230个独特基因，包括204个蛋白质编码基因（PCG）、21个转运RNA基因、3个核糖体RNA基因、1个转运信使RNA基因和1个非编码RNA基因。在204个PCG中，ccsA基因被一个内含子中断。与高等植物cpDNA典型的四分体结构（由小单拷贝和大单拷贝单元分隔的一对反向重复序列）不同，该红藻的完整cpDNA非常紧凑，不包含反向重复序列，只有一个由16S、23S和5S rRNA基因组成的rRNA基因簇拷贝。基因区域占整个cpDNA基因组的83.7%，cpDNA的G+C含量为27.4%。该红藻cpDNA的低G+C含量主要是由PCG和非编码区域的高A+T含量造成的。对15种红藻完整cpDNA的系统发育分析表明，该红藻与大型藻类密切相关。该红藻的完整cpDNA为红藻进一步的系统发育和进化分析提供了重要的DNA分子数据。
