Wu Zhiqiang, Gu Cuihua, Tembrock Luke R, Sun Cheng
Department of Biology, Colorado State University, Fort Collins, CO, USA.
School of Landscape and Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, PR China.
Mitochondrial DNA B Resour. 2016 Apr 18;1(1):291-292. doi: 10.1080/23802359.2016.1166082.
We report the complete chloroplast DNA (cpDNA) of (Brassicaceae), a less studied relative of by employing next-generation sequencing reads and assembly. The length of the closed circular cpDNA is 154,604 bp with a typical quadripartite structure. The genome is composed of one large single copy and one small single copy regions of 84,209 bp and 17,871 bp, respectively, and separated by a pair of inverted repeats of 26,262 bp in length. The overall GC content is 36.35% and the GC content of the LSC, IRs and SSC regions are 34.12%, 42.30% and 29.38%, separately. The gene content and the number for are the same as other published species in Brassicaceae with 112 annotated known unique genes including 78 protein-coding genes, 30 tRNA genes and four rRNA genes. The complete cpDNA of will provide valuable molecular resources for further phylogenetic and evolutionary analysis in the model genus.
我们通过利用二代测序读数和从头组装技术，报道了十字花科中一个较少被研究的物种——[物种名称]的完整叶绿体DNA（cpDNA）。闭合环状cpDNA的长度为154,604 bp，具有典型的四分体结构。该基因组由一个84,209 bp的大单拷贝区和一个17,871 bp的小单拷贝区组成，二者由一对长度为26,262 bp的反向重复序列隔开。总体GC含量为36.35%，大单拷贝区、反向重复序列区和小单拷贝区的GC含量分别为34.12%、42.30%和29.38%。[物种名称]的基因组成和数量与十字花科其他已发表物种相同，有112个注释的已知独特基因，包括78个蛋白质编码基因、30个tRNA基因和4个rRNA基因。[物种名称]的完整cpDNA将为模式[属名称]属的进一步系统发育和进化分析提供有价值的分子资源。
