……的线粒体基因组分析
Mitochondrial genome analysis of .
作者信息
Yuan Xiao-Long, Zhang Zhong-Feng, Liu Xin-Min, Du Yong-Mei, Zhang Peng, Hou Xiao-Dong
机构信息
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
出版信息
Mitochondrial DNA B Resour. 2016 Feb 10;1(1):43-44. doi: 10.1080/23802359.2015.1137814.
is a species of cichlid endemic and prefers sheltered bays or vegetated substrates. In this study, the mitochondrial genome of was completed using PCR and Sanger sequencing method. The genome is a circular molecule with the length of 16 576 bp and contains 13 protein-coding genes (PCGs), 22 transfer tRNA genes and two ribosomal RNA genes. Except gene, the other protein-coding genes are started with ATG. The average A + T content is 54.1%. Gene overlaps have been found at four junctions and have involved a total of 26 bp. Compared with other cichlid species, the genome exhibits high similarity in both the genome size and the genome structure. ML phylogenetic analysis was implemented with other 20 related species to verity the genetic relationship of .
是一种特有的丽鱼科鱼类，偏好隐蔽的海湾或有植被的基质。在本研究中，使用聚合酶链式反应（PCR）和桑格测序法完成了该物种的线粒体基因组测序。该基因组是一个长度为16576碱基对的环状分子，包含13个蛋白质编码基因（PCGs）、22个转运RNA基因和两个核糖体RNA基因。除了基因外，其他蛋白质编码基因均以ATG起始。平均A+T含量为54.1%。在四个连接处发现了基因重叠，总共涉及26个碱基对。与其他丽鱼科物种相比，该基因组在基因组大小和基因组结构上均表现出高度相似性。利用其他20个相关物种进行了最大似然法（ML）系统发育分析，以验证该物种的遗传关系。
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