Shikano Takahito, Guo Baocheng, Vukić Jasna, Šanda Radek, Merilä Juha
Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, Helsinki, Finland.
Department of Ecology, Faculty of Science, Charles University of Prague, Prague, Czech Republic.
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):66-67. doi: 10.1080/23802359.2015.1137826.
The complete mitochondrial genome of the Greek nine-spined stickleback was obtained using Illumina high-throughput sequencing of genomic DNA. The genome was 16 713 bp long, and contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region. The arrangement of the genes was identical to that of other Gasterosteidae fishes. However, the control region of contained three copies of imperfect repeated sequences (72-78 bp in single motifs), while and have one or two copies. Nucleotide identity between and three other species across all the 37 genic regions was 93.0% to 95.5%.
利用Illumina对基因组DNA进行高通量测序，获得了希腊九刺鱼的完整线粒体基因组。该基因组长度为16713bp，包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个控制区。基因排列与其他刺鱼科鱼类相同。然而，该控制区包含三个不完美重复序列拷贝（单基序中为72 - 78bp），而其他物种有一个或两个拷贝。在所有37个基因区域中，该物种与其他三种刺鱼科物种之间的核苷酸同一性为93.0%至95.5%。
