Hao Zhen Lin, Yang Li Meng, Zhan Yao Yao, Tian Ying, Mao Jun Xia, Wang Luo, Chang Ya Qing
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, Liaoning, China.
Mitochondrial DNA B Resour. 2016 Mar 28;1(1):220-221. doi: 10.1080/23802359.2016.1155421.
The complete mitochondrial (mt) genome of the Neptune whelk, , was determined using genome walking techniques in this study. The total length of the mt genome sequence of was 15 256 bp, including 13 protein-coding genes, 21 transfer RNA genes and 2 ribosomal RNA genes. The overall composition of the mitogenome was estimated to be 30.85% for A, 38.59% for T, 15.15% for C and 15.40% for G, indicating that an A + T (69.44%)-rich feature occurs in the mitogenome. The phylogenetic relationships of 11 mollusc species were constructed based on the complete mtDNA sequences by the neighbour-joining method using MEGA 5.0 software (MEGA Inc., Englewood, NJ).
在本研究中，利用基因组步移技术测定了海王星蛾螺的完整线粒体（mt）基因组。海王星蛾螺的线粒体基因组序列全长15256 bp，包括13个蛋白质编码基因、21个转运RNA基因和2个核糖体RNA基因。线粒体基因组的总体组成估计为：A占30.85%，T占38.59%，C占15.15%，G占15.40%，表明海王星蛾螺的线粒体基因组具有富含A+T（69.44%）的特征。使用MEGA 5.0软件（MEGA公司，新泽西州恩格尔伍德），通过邻接法基于完整的线粒体DNA序列构建了11种软体动物的系统发育关系。
