Department of Marine Organism Taxonomy & Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, P. R. China.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, P. R. China.
Mol Biol Rep. 2022 Sep;49(9):9065-9069. doi: 10.1007/s11033-022-07639-z. Epub 2022 Jul 9.
The Neptune whelk, Neptunea cumingii, is an economic gastropod endemic to the East Asia Warm Temperate Biotic Subregion. The study of the development in microsatellite markers maybe beneficial for assessing the genetic diversity and conservation of resources on this dwindling species.
The microsatellite markers were constructed and characterized through Illumina high-throughput sequencing and capillary electrophoresis techniques. Eleven polymorphic microsatellite loci were screened and validated. The observed heterozygosity and expected heterozygosity of each locus showed a range from 0.0600 to 0.6508 and from 0.7380 to 0.9375, respectively. The average Shannon's information index and polymorphism information content were 2.0828 and 0.8325, respectively. Deviation from all loci was generated from Hardy-Weinberg equilibrium.
The 11 microsatellite markers developed in this study show polymorphic status. These markers are expected to be highly informative for further analysis of genetic diversity in N. cumingii.
Neptunea cumingii 是东亚暖温带生物亚区特有的经济腹足类动物。研究微卫星标记的发育可能有助于评估该濒危物种的遗传多样性和资源保护。
通过 Illumina 高通量测序和毛细管电泳技术构建和表征了微卫星标记。筛选和验证了 11 个多态性微卫星位点。每个位点的观测杂合度和期望杂合度范围分别为 0.0600 至 0.6508 和 0.7380 至 0.9375。平均香农信息指数和多态信息含量分别为 2.0828 和 0.8325。所有位点的偏离均来自 Hardy-Weinberg 平衡。
本研究开发的 11 个微卫星标记呈现多态性状态。这些标记有望为进一步分析 N. cumingii 的遗传多样性提供高度信息。
