Yang Jiacheng, Zhu Jianye, Shi Ling, Li Zhuo, Zhang Dandan, Tian Ying, Hao Zhenlin
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, PR China.
Dalian Shell Museum, Dalian, PR China.
Mitochondrial DNA B Resour. 2020 Jan 8;5(1):434-435. doi: 10.1080/23802359.2019.1704183.
The complete mitochondrial (mt) genome of was determined using genome walking techniques in this study. The total length of the mt genome sequence of was 16,551 bp, including 13 protein-coding genes, 23 transfer RNA genes, and 2 ribosomal RNA genes. The overall composition of the mitogenome was estimated to be 33.1% for A, 35.3% for T, 13.7% for C, and 19.9% for G, respectively, indicating that an A + T (68.4%)-rich feature occurs in the mitogenome. The phylogenetic relationships of 12 mollusk species were constructed based on the complete mtDNA sequences by the neighbor-joining method using MEGA version 7.0 and DNAMAN version 6.0 software.
在本研究中,使用基因组步移技术测定了[物种名称]的完整线粒体(mt)基因组。[物种名称]的线粒体基因组序列全长为16,551 bp,包括13个蛋白质编码基因、23个转运RNA基因和2个核糖体RNA基因。估计该线粒体基因组的总体组成分别为:A占33.1%,T占35.3%,C占13.7%,G占19.9%,这表明[物种名称]的线粒体基因组具有富含A+T(68.4%)的特征。基于完整的线粒体DNA序列,使用MEGA 7.0版本和DNAMAN 6.0版本软件,通过邻接法构建了12种软体动物物种的系统发育关系。