Wang Jinlong, Zou Li, Jiang Guomin, Zeng Ming, Li Chuanwu, Wu Yuanan, Wang Dongwu, Liu Mingqiu, Liu Li
Fisheries Research Institute of Hunan Province, Changsha, Hunan, P.R. China.
Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, Hunan, P.R. China.
Mitochondrial DNA B Resour. 2016 Jun 22;1(1):428-429. doi: 10.1080/23802359.2016.1180552.
The complete mitochondrial genome of the hybrid of (♀)× (♂) was characterized first in this study. The total length of the genome was identical to the female parent as 16,581 bp, and the overall base composition was 31.80% A, 24.85% T, 27.55% C and 15.80% G, with a slight A + T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light-strand replication). This study discovered the 99.70% sequence identity between the hybrid and its female parent, which confirmed the maternal inheritance pattern followed by the mitochondrial genome of the hybrid. However, the sequence alignment of mitochondrial genomes between the hybrid and its female parent revealed a total of 47 variable sites in 15 genes or regions, especially 25 sense mutations in 6 PCGs. The complete mitochondrial genome sequence of the hybrid may provide an important dataset for further study in mitochondrial inheritance mechanism.
本研究首次对(♀)×(♂)杂交种的完整线粒体基因组进行了表征。该基因组的总长度与母本相同,为16,581 bp,总体碱基组成为31.80%A、24.85%T、27.55%C和15.80%G,具有轻微的A+T偏向性。它包含13个蛋白质编码基因(PCGs)、22个转移RNA基因、2个核糖体RNA基因和2个主要非编码区域(控制区和轻链复制起点)。本研究发现杂交种与其母本之间的序列同一性为99.70%,这证实了杂交种线粒体基因组遵循母系遗传模式。然而,杂交种与其母本线粒体基因组的序列比对显示,在15个基因或区域中共有47个可变位点,特别是在6个PCGs中有25个有义突变。杂交种的完整线粒体基因组序列可能为进一步研究线粒体遗传机制提供重要的数据集。