Coates Brad S, Abel Craig A
United States Department of Agriculture, Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, Ames, IA, USA.
Department of Entomology, Iowa State University, Ames, IA, USA.
Mitochondrial DNA B Resour. 2016 Jul 10;1(1):487-488. doi: 10.1080/23802359.2016.1192499.
The complete 15,553 bp mitochondrial genome of the western bean cutworm, , (Lepidoptera: Noctuidae) was assembled from paired end Illumina HiSeq2500 read data. Annotation showed 13 predicted protein coding genes (PCGs), 22 tRNAs and 2 rRNAs have an order and orientation typical of insect mitochondrial genomes, and the derived rearrangement of tRNA-Met, -Ile and -Gln upstream of 2 as in other Lepidoptera. A 79.3% A + T content resulted in a bias for codons with A or T in the 3rd position, and prevalence of synonymous substitutions suggest the effects of purifying selection on the mitochondrial genome sequence. Two microsatellite repeat motifs, (CA)(AT) and (AT), are respectively located in intergenic spaces between tRNA-Glu and -Phe and tRNA-Leu and 16S rRNA. Mitochondrial phylogenomics was able to resolve sub-families within the Noctuidae, and suggest analogous analyses may be applicable across other lepidopteran Families.
西部豆夜蛾(Lepidoptera: Noctuidae)完整的15,553 bp线粒体基因组是根据成对末端Illumina HiSeq2500读取数据组装而成。注释显示,13个预测的蛋白质编码基因(PCG)、22个tRNA和2个rRNA具有昆虫线粒体基因组典型的顺序和方向,并且tRNA-Met、-Ile和-Gln在2个上游的衍生重排与其他鳞翅目昆虫相同。79.3%的A + T含量导致密码子在第3位偏向于含有A或T,同义替换的普遍性表明纯化选择对线粒体基因组序列的影响。两个微卫星重复基序(CA)(AT)和(AT)分别位于tRNA-Glu和-Phe以及tRNA-Leu和16S rRNA之间的基因间隔区。线粒体系统发育基因组学能够解析夜蛾科内的亚科,并表明类似的分析可能适用于其他鳞翅目科。