Coates Brad S, Sumerford Douglas V, Hellmich Richard L, Lewis Leslie C
USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, c/o Insectary, Iowa State University, Ames, Iowa, 50011.
Int J Biol Sci. 2005;1(1):13-8. doi: 10.7150/ijbs.1.13. Epub 2005 Jan 5.
Contiguous 14,535 and 14,536 nt near complete mitochondrial genome sequences respectively were obtained for Ostrinia nubilalis and Ostrinia furnicalis. Mitochondrial gene order was identical to that observed from Bombyx. Sequences comparatively showed 186 substitutions (1.3% sequence divergence), 170 CDS substitutions (131 at 3(rd) codon positions), and an excess of transition mutation likely resulting by purifying selection (d(N)/d(S) = omega congruent with 0.15). Overall substitution rates were significantly higher at 4-fold (5.2%) compared to 2-fold degenerate codons (2.6%). These are the 3(rd) and 4(th) lepidopteran mitochondrial genome reference sequences in GenBank and useful for comparative mitochondrial studies.
分别获得了欧洲玉米螟和亚洲玉米螟接近完整的线粒体基因组序列，长度分别为14,535和14,536个核苷酸。线粒体基因排列顺序与家蚕中观察到的相同。序列比较显示有186个替换（序列差异为1.3%），170个编码区替换（131个在第三密码子位置），并且由于纯化选择可能导致转换突变过量（d(N)/d(S)=ω≈0.15）。与二倍简并密码子（2.6%）相比，四倍简并密码子的总体替换率显著更高（5.2%）。这些是GenBank中第三和第四个鳞翅目线粒体基因组参考序列，对线粒体比较研究很有用。
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