Aunins Aaron William, Nelms David L, Hobson Christopher S, King Timothy L
Leetown Science Center, Aquatic Ecology Branch, Natural Systems Analysts, Inc, Kearneysville, WV, USA.
United States Geological Survey, Virginia Water Science Center, Richmond, VA, USA.
Mitochondrial DNA B Resour. 2016 Nov 21;1(1):560-563. doi: 10.1080/23802359.2016.1174086.
The mitochondrial genomes of three North American stygobiont amphipods , and collected from Caroline County, VA, were sequenced using a shotgun sequencing approach on an Illumina NextSeq500 (Illumina Inc., San Diego, CA). All three mitogenomes displayed 13 protein-coding genes, 22 tRNAs and two rRNAs typical of metazoans. While and displayed identical gene orders similar to the pancrustacean ground pattern, displayed a transposition of the - genes to after . In addition, a short gene, longer gene and large inverted repeat within the Control Region distinguished from and . Overall, it appears that gene order varies considerably among amphipods, and the addition of these mitogenomes to the existing sequenced amphipod mitogenomes will prove useful for characterizing evolutionary relationships among various amphipod taxa, as well as investigations of the evolutionary dynamics of the mitogenome in general.
对从弗吉尼亚州卡罗琳县采集的三种北美洞穴生境双足节肢动物(此处未提及具体物种名,分别用 、 和 指代)的线粒体基因组,采用鸟枪法测序法在Illumina NextSeq500测序仪(Illumina公司,加利福尼亚州圣地亚哥)上进行测序。所有三个线粒体基因组均显示出后生动物典型的13个蛋白质编码基因、22个tRNA和两个rRNA。虽然 和 显示出与泛甲壳动物基本模式相似的相同基因顺序,但 显示出 - 基因转位到 之后。此外,控制区内一个短的 基因、较长的 基因和大的反向重复序列使 与 和 区分开来。总体而言,双足节肢动物之间的基因顺序似乎差异很大,将这三个线粒体基因组添加到现有的已测序双足节肢动物线粒体基因组中,将有助于表征各种双足节肢动物分类群之间的进化关系,以及一般情况下线粒体基因组进化动态的研究。