Yi Dong-Keun, Kim Ki-Joong
School of Life Sciences, Korea University, Seoul, Korea.
Mitochondrial DNA B Resour. 2016 Sep 5;1(1):627-629. doi: 10.1080/23802359.2016.1214546.
The nucleotide sequence of the two chloroplast (cp) genomes from and are the first to be completed in genus of family Paulowniaceae. The structure of two Paulownia cp genomes shows similar characteristic with general cp genome of angiosperms. The lengths of two cp genomes are 154,545 bp and 154,540 bp, respectively. The cp genomes are divided into LSC region (85,241 bp and 85,236 bp) and SSC region (17,736 bp and 17,736 bp) by two IR regions (25,784 bp and 25,784 bp). Both of two cp genomes contain 113 genes (79 protein coding genes, 30 tRNA genes and 4 rRNA genes), eight protein-coding genes, seven tRNA genes and four rRNA genes duplicated in the IR regions. Similar to the general cp genome of angiosperms, 18 of the genes in the two cp genomes have one or two introns. The overall A-T contents of two genomes are 62.0% which is similar with general angiosperms. The A-T content in the non-coding (64.6%) is higher than in the coding (60.1%) regions. Seventy-one and seventy simple sequence repeat (SSR) loci were identified in the and cp genomes, respectively. In phylogenetic analysis, genus shows closed relationship with of Orobanchaceae.
来自泡桐科泡桐属的两个叶绿体（cp）基因组的核苷酸序列是该属中最先完成测序的。两种泡桐cp基因组的结构与被子植物的一般cp基因组具有相似特征。两个cp基因组的长度分别为154,545 bp和154,540 bp。cp基因组被两个反向重复（IR）区域（25,784 bp和25,784 bp）分为大单拷贝（LSC）区域（85,241 bp和85,236 bp）和小单拷贝（SSC）区域（17,736 bp和17,736 bp）。两个cp基因组均包含113个基因（79个蛋白质编码基因、30个tRNA基因和4个rRNA基因），其中8个蛋白质编码基因、7个tRNA基因和4个rRNA基因在IR区域中重复。与被子植物的一般cp基因组相似，两个cp基因组中有18个基因含有一个或两个内含子。两个基因组的总体A-T含量为62.0%，与一般被子植物相似。非编码区（64.6%）的A-T含量高于编码区（60.1%）。在两个泡桐cp基因组中分别鉴定出71个和70个简单序列重复（SSR）位点。在系统发育分析中，泡桐属与列当科的肉苁蓉属显示出密切的关系。
