Xing Yutong, Mao Xiuguang
Institute of Estuarine and Coastal Research, East China Normal University, Shanghai, China.
Mitochondrial DNA B Resour. 2016 Dec 23;1(1):964-965. doi: 10.1080/23802359.2016.1266707.
The Thomas's horseshoe bat () is restricted to southeast Asia and few studies have been conducted on this species. Here we characterized the complete mitochondrial genome of using next-generation sequencing and Sanger sequencing. The whole mitogenome is 16,899 bp in length and contains 22 tRNA, 2 rRNA, 13 protein-coding genes and a non-coding control region. The tissue used in this study was taken from a sample collected 13 years ago and the genomic DNA was seriously degraded. Our study adds to a growing number of studies indicating that next-generation sequencing is powerful in generating genetic/genomic resources from museum samples.
托氏菊头蝠()仅分布于东南亚,针对该物种的研究较少。在此,我们利用二代测序和桑格测序对托氏菊头蝠的完整线粒体基因组进行了特征分析。整个线粒体基因组长度为16,899 bp,包含22个tRNA、2个rRNA、13个蛋白质编码基因和一个非编码控制区。本研究中使用的组织取自13年前采集的一个样本,基因组DNA严重降解。我们的研究为越来越多的研究增添了内容,这些研究表明二代测序在从博物馆样本中生成遗传/基因组资源方面很强大。