基于系统发育分析的日本鲐线粒体全基因组分析
Complete mitochondrial genome analysis of Japanese Spanish mackerel () with phylogenetic consideration.
作者信息
Jin Yan, Ouyang Longling, Zheng Hongxiang, Lin Yu, Zhang Menghan, Li Shengfa, Cheng Jiahua
机构信息
Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.
出版信息
Mitochondrial DNA B Resour. 2017 Feb 2;2(1):56-57. doi: 10.1080/23802359.2017.1280700.
The complete mitochondrial DNA (mtDNA) of Japanese Spanish mackerel () was cloned and sequenced. The total length of the mitochondrial genome is 16,646 bp with an accession number KY228987. Thirty-seven genes are identified in total, including 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a putative D-loop region. Among these genes, 9 are encoded on the light strand, while others are encoded on the heavy strand. The overall base composition of mitogenome is 28.39% for A, 16.14% for G, 26.52% for T, 28.96% for C, respectively, with a slight higher A + T content (54.91%). The phylogeny analysis based on 18 COI amino acid sequences suggested that . and the other two species from (Scombridae) formed a cluster apart from the one comprising other genus from Scombridae. The complete mitogenome may shed light on the future study of genetic mechanism of .
对日本鲭（）的完整线粒体DNA（mtDNA）进行了克隆和测序。线粒体基因组全长16646 bp，登录号为KY228987。共鉴定出37个基因，包括13个蛋白质编码基因、22个转移RNA（tRNA）基因、2个核糖体RNA（rRNA）基因和一个假定的D环区域。在这些基因中，9个基因由轻链编码，其他基因由重链编码。线粒体基因组的总体碱基组成为：A占28.39%，G占16.14%，T占26.52%，C占28.96%，A+T含量略高（54.91%）。基于18个细胞色素氧化酶亚基I（COI）氨基酸序列的系统发育分析表明，。与鲭科（Scombridae）的其他两个物种形成一个聚类，与鲭科其他属的聚类分开。完整的线粒体基因组可能为未来对的遗传机制研究提供线索。
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