Jondeung Amnuay, Karinthanyakit Wirangrong
Department of Genetics, Kasetsart University, Bangkok, Thailand.
Mitochondrial DNA. 2010 Apr;21(2):36-47. doi: 10.3109/19401731003622529.
In order to support studies of short mackerel population genetic structure in the Gulf of Thailand and phylogenetic relationships, the mitochondrial genome of the short mackerel, Rastrelliger brachysoma, has recently been determined by a partial cloning technique, long PCR with three pairs of newly designed primers and primer walking sequencing. The complete mitochondrial genome is 16,539 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) and a control region (CR) as in other bony fishes. Within the 845-bp CR, we identified several conserved motifs. The phylogeny obtained by Bayesian analyses based on two nucleotide datasets corresponding to the cytb and nd2 mitochondrial genes strongly support the inclusion of R. brachysoma within the monophyletic tribe of Scombrini in the family Scombridae. The obtained phylogeny also reveals high-statistical support for the existence of two distinct groups indicating that Scombroidei and Xiphioidei are two separate suborders.
为了支持对泰国湾短鳍小沙丁鱼种群遗传结构及其系统发育关系的研究，近期通过部分克隆技术、利用三对新设计的引物进行长片段PCR以及引物步移测序，确定了短鳍小沙丁鱼（Rastrelliger brachysoma）的线粒体基因组。完整的线粒体基因组长度为16,539 bp，与其他硬骨鱼类一样，包含37个线粒体基因（13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因）以及一个控制区（CR）。在845 bp的控制区内，我们鉴定出了几个保守基序。基于细胞色素b（cytb）和线粒体基因nd2的两个核苷酸数据集进行贝叶斯分析所获得的系统发育关系，有力地支持了短鳍小沙丁鱼归属于鲭科鲭族的单系群。所获得的系统发育关系还显示出高度的统计学支持，表明鲭亚目和剑鱼亚目是两个独立的亚目。
