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基于线粒体基因组的广西石鸡(鸟纲,鸡形目)全序列克隆及生物信息学分析

Complete sequence cloning and bioinformatics analysis of the chukar partridge (, Aves, Galliformes) in Guangxi base on mitochondrial genome.

作者信息

Zhang Yanfang, Xie Zhixun, Deng Xianwen, Xie Zhiqin, Xie Liji, Fan Qing, Luo Sisi, Liu Jiabo, Huang Li, Huang Jiaoling, Zeng Tingting, Wang Sheng

机构信息

Department of Biotechnology, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, China.

出版信息

Mitochondrial DNA B Resour. 2017 Jun 16;2(1):361-362. doi: 10.1080/23802359.2017.1339215.

Abstract

The objective of this study was to obtain the complete mitochondrial DNA sequence of chukar partridge, and to provide reference data for protection and utilization of these resources of chukar partridge. The complete mitochondrial genome sequence of the China chukar partridge was measured by PCR-based methods and analysed in detail. Our research findings reveal that the entire mitochondrial genome of the chukar partridge is a circular molecule consisting of 16,688 bp (GenBank accession number: KY829450). The contents of A, T, C, and G in the mitochondrial genome were found to be 30.44%, 24.43%, 31.57%, and 13.56%, respectively. The complete mitochondrial genome of the chukar partridge has a typical structure, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one control region (D-loop region). This complete mitochondrial genome sequence provides essential information in understanding phylogenetic relationships among Galliformes mitochondrial genomes.

摘要

本研究的目的是获得石鸡的完整线粒体DNA序列,为石鸡资源的保护和利用提供参考数据。采用基于PCR的方法测定了中国石鸡的完整线粒体基因组序列,并进行了详细分析。我们的研究结果表明,石鸡的整个线粒体基因组是一个由16,688 bp组成的环状分子(GenBank登录号:KY829450)。线粒体基因组中A、T、C和G的含量分别为30.44%、24.43%、31.57%和13.56%。石鸡的完整线粒体基因组具有典型的结构,包括13个蛋白质编码基因、两个rRNA基因、22个tRNA基因和一个控制区(D-loop区)。这个完整的线粒体基因组序列为理解鸡形目线粒体基因组之间的系统发育关系提供了重要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4521/7800480/f8639ed3048f/TMDN_A_1339215_F0001_B.jpg

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