Wang Min, Wang Ling, Wang Cheng-Qiao, Wan Sheng-Hao, Wang Hu, Sun Jie-Yu, Tang Jian-Qiang, Zhang Xiao-Chun, Zhang Hai-Jun, Li Jun, Peng Shu-Ying
Human and Animal Genetics Laboratory, School of Life Science, Huaibei Normal University, Huaibei, Anhui, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Oct 1;4(2):3298-3300. doi: 10.1080/23802359.2019.1673232.
Partridge Shank chicken is a valuable broiler breed in China. The first complete mitochondrial DNA (mtDNA) sequence of Partridge Shank chicken had been obtained using PCR amplification, sequencing and assembling. The complete mitochondrial genome was 16,788 bp in length, with the base composition of 30.2% for A, 23.7% for T, 32.5% for C and 13.5% for G. It exhibited the typical mitochondrial structure, including 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a non-coding control region (D-loop region). The phylogenetic tree construced with maximum-likelihood (ML) method based on the complete chicken mitochondrial genomes showed that the 26 chicken breeds could be divided into two groups.
鹧鸪腿鸡是中国一种有价值的肉鸡品种。利用PCR扩增、测序和组装技术,已获得了鹧鸪腿鸡的首个完整线粒体DNA(mtDNA)序列。完整的线粒体基因组长度为16,788 bp,碱基组成为:A占30.2%,T占23.7%,C占32.5%,G占13.5%。它呈现出典型的线粒体结构,包括2个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和一个非编码控制区(D-loop区)。基于完整鸡线粒体基因组,采用最大似然法构建的系统发育树表明,26个鸡品种可分为两组。