Choquet Marvin, Alves Monteiro Homère J, Bengtsson-Palme Johan, Hoarau Galice
Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Mitochondrial DNA B Resour. 2017 Aug 6;2(2):506-507. doi: 10.1080/23802359.2017.1361357.
, a marine planktonic copepod, is a keystone species in the Arctic Ocean. In this study, we shotgun sequenced the total DNA of one individual, using the NextSeq Illumina platform, in order to determine its mitochondrial genome sequence. We successfully assembled and annotated this 20,674 bp long sequence, which included 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. Common gene-coding regions of 19 other species were used to reconstruct a phylogenetic tree, using mitogenomes of the phylogenetically closest copepods available. The new resource described here constitutes a tool of interest for better understanding the structure and dynamics of populations.
一种海洋浮游桡足类动物，是北冰洋的关键物种。在本研究中，我们使用Illumina NextSeq平台对一个个体的总DNA进行了鸟枪法测序，以确定其线粒体基因组序列。我们成功组装并注释了这个长度为20,674 bp的序列，其中包括13个蛋白质编码基因、2个rRNA基因和22个tRNA基因。利用19个其他物种的常见基因编码区域，结合现有系统发育关系最密切的桡足类动物的有丝分裂基因组，重建了系统发育树。这里描述的新资源是一个有助于更好地理解种群结构和动态的有趣工具。
