Ramos A A, Weydmann A, Cox C J, Canário A V M, Serrão E A, Pearson G A
CCMAR-Centre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139 Faro, Portugal.
Institute of Oceanography, University of Gdansk, Piłsudskiego 46, 81-378 Gdynia, Poland; Marine Ecology Department, Institute of Oceanology, Polish Academy of Sciences, Powstancow Warszawy 55, 81-712 Sopot, Poland.
Mar Genomics. 2015 Oct;23:27-9. doi: 10.1016/j.margen.2015.03.014. Epub 2015 Apr 8.
The copepod Calanus glacialis plays a key role in the Arctic pelagic ecosystem. Despite its ecological importance and ongoing climate changes, limited knowledge at the genomic level has hindered the understanding of the molecular processes underlying environmental stress responses and ecological adaptation. Transcriptome data was generated from an experiment with C. glacialis copepodite (CV) subjected to five different temperatures. We obtained a total of 512,352 high-quality 454 pyrosequencing reads, which were assembled into 55,562 contigs distributed in 128 KEGG pathways. Functional analysis revealed numerous genes related to diverse biological functions and processes, including members of all major conserved signaling pathways. Comparative analysis of acclimated individuals to experimental temperatures has provided information about gene variations observed in several pathways (e.g. genes involved in energy, lipid and amino acid metabolism were shown to be down-regulated with increasing temperatures). These mRNA sequence resources will facilitate further studies on genomics and physiology-driven molecular processes in C. glacialis and related species.
桡足类的冰川哲水蚤在北极海洋生态系统中起着关键作用。尽管其具有生态重要性且气候变化持续存在，但基因组水平上的有限知识阻碍了对环境应激反应和生态适应背后分子过程的理解。转录组数据来自对处于五种不同温度下的冰川哲水蚤桡足幼体（CV）进行的一项实验。我们总共获得了512,352条高质量的454焦磷酸测序读数，这些读数被组装成55,562个重叠群，分布在128条KEGG通路中。功能分析揭示了许多与多种生物学功能和过程相关的基因，包括所有主要保守信号通路的成员。对适应实验温度的个体进行的比较分析提供了在几个通路中观察到的基因变异信息（例如，参与能量、脂质和氨基酸代谢的基因显示随着温度升高而下调）。这些mRNA序列资源将有助于进一步研究冰川哲水蚤及相关物种的基因组学和生理驱动的分子过程。
