Zhang Guosong, Liang Xia, Zhang Jiajia, Li Jie, Yin Shaowu
College of Life Sciences, Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, Nanjing Normal University, Nanjing, Jiangsu, China.
Department of Life Science, Heze University, Heze, Shandong, China.
Mitochondrial DNA B Resour. 2017 Aug 30;2(2):577-578. doi: 10.1080/23802359.2017.1372711.
In this work, we reported the complete mitochondrial DNA sequence of the hybrid of (♂)× (♀), which was obtained by artificial hybridization. The total length of the mitochondrial genome is 16,527 bp, with the base composition of 30.86% for A, 25.56% for T, 28.20% for C, and 15.38% for G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the teleosts. All the protein initiation codons are ATG, except for COX1 that begins with GTG. The complete mitogenome of the hybrid provides an important data set for the study in mitochondrial inheritance mechanism. Both the termination-associated sequence and critical central conserved sequences (CSB-D, CSB-E, and CSB-F) were also detected.
在本研究中,我们报道了通过人工杂交获得的(♂)×(♀)杂交种的完整线粒体DNA序列。线粒体基因组全长16,527 bp,碱基组成为:A占30.86%,T占25.56%,C占28.20%,G占15.38%。它包含两个核糖体RNA基因、13个蛋白质编码基因、22个转移RNA基因和一个主要的非编码控制区(D环区)。这些基因的排列与硬骨鱼中发现的排列相同。除了以GTG起始的COX1外,所有蛋白质起始密码子均为ATG。该杂交种的完整线粒体基因组为线粒体遗传机制的研究提供了重要的数据集。还检测到了终止相关序列和关键的中央保守序列(CSB-D、CSB-E和CSB-F)。