Zhu Kecheng, Zhang Dongling, Wei Jinfen, Huang Guiju, Guo Yihui, Jiang Song, Yu Dahui
a Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture , South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , China .
b Key Laboratory of Healthy Mariculture for the East China Sea , Ministry of Agriculture, Fisheries College, Jimei University , Xiamen , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1968-9. doi: 10.3109/19401736.2014.971310. Epub 2014 Oct 16.
In this study, we reported the complete mitochondrial DNA sequence of the hybrid grouper Epinephelus fuscoguttatus (♀)×Epinephelus lanceolatus (♂). The full-length of the mitochondrial genome consisted of a 16,644 bp fragment, with the base composition of A (29.21%), C (26.84%), G (15.65%) and T (28.29%). It contained 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes, and a major non-coding control region (D-loop region). The composition and order of these genes were identical to most other vertebrates. All the protein initiation codons were ATG, except that COX1 began with GTG and ATP-6 was not determined. The complete mitogenome of the hybrid E. fuscoguttatus (♀)×E. lanceolatus (♂) provided an important data set for the study in genetic mechanism of the hybridization.
在本研究中,我们报道了杂交石斑鱼棕点石斑鱼(♀)×鞍带石斑鱼(♂)的完整线粒体DNA序列。线粒体基因组全长由一个16,644bp的片段组成,碱基组成为A(29.21%)、C(26.84%)、G(15.65%)和T(28.29%)。它包含2个rRNA基因、13个蛋白质编码基因、22个tRNA基因和一个主要的非编码控制区(D环区)。这些基因的组成和顺序与大多数其他脊椎动物相同。所有蛋白质起始密码子均为ATG,但COX1以GTG起始且ATP-6未确定。杂交棕点石斑鱼(♀)×鞍带石斑鱼(♂)的完整线粒体基因组为杂交遗传机制的研究提供了重要数据集。