Sheibaninia Atena, Nejatkhah Manavi Parisa, Houshmand Massoud
Department of Marine Biology, Faculty of Marine Science and Technology, Islamic Azad University, Tehran, Iran.
Department of Medical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Mitochondrial DNA B Resour. 2017 Sep 26;2(2):676-678. doi: 10.1080/23802359.2017.1357434.
Silver pomfret fish (Stromateidae) is a family of Perciformes order. One species of this family is known as silver pomfret and lives in different parts of the Persian Gulf and Gulf of Oman. Therefore, the genetic relationship between its populations is so important. In this study, eight samples of were collected from four locations involved: Emam Khomeyni, Bushehr and Bandar Abbas in Persian Gulf and Chabahar in Gulf of Oman. Cytochrome b gene and D-loop region of mitochondrial DNA on these samples are analyzed. These genes are carried out using PCR with specific mtDNA primers and sequenced. Then, the evolutionary history was inferred by using the maximum-likelihood method. Evolutionary analyses were conducted in MEGA7. According to the phylogenetic trees, with translocations in Persian Gulf to Gulf of Oman, similarity decreases slowly. Chabahar1 sample in phylogenetic tree of cytochrome b is phylogenetically near China Sea samples. The reason may be migration from ocean to gulf throughout the centuries and Chabahar is in Gulf of Oman and locationally near the Arabian Sea and Indian Ocean. In D-loop region, results are similar to cytochrome b excepting one sample (Emam Khomeyni1). D-Loop in samples shows modification more than cytochrome b for the reason absence of protein product.
银鲳鱼（鲳科）属于鲈形目。该科的一个物种被称为银鲳，生活在波斯湾和阿曼湾的不同区域。因此，其种群之间的遗传关系非常重要。在本研究中，从四个地点采集了八个样本，这些地点包括：波斯湾的霍梅尼港、布什尔和阿巴斯港以及阿曼湾的恰巴哈尔。对这些样本的线粒体DNA的细胞色素b基因和D环区域进行了分析。使用特定的线粒体DNA引物通过聚合酶链反应（PCR）对这些基因进行扩增并测序。然后，使用最大似然法推断进化历史。在MEGA7中进行进化分析。根据系统发育树，随着从波斯湾到阿曼湾的迁移，相似性缓慢降低。细胞色素b系统发育树中的恰巴哈尔1样本在系统发育上与中国海样本接近。原因可能是几个世纪以来从海洋到海湾的迁徙，而恰巴哈尔位于阿曼湾，地理位置靠近阿拉伯海和印度洋。在D环区域，除了一个样本（霍梅尼港1）外，结果与细胞色素b相似。样本中的D环显示出比细胞色素b更多的变异，原因是其没有蛋白质产物。
