Li Yuan, Gao Tianxiang, Song Na
a Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):684-5. doi: 10.3109/19401736.2014.913142. Epub 2014 Apr 29.
Long PCR and primer walking methods are employed for determining the complete mitochondrial genome sequence of Pampus argenteus. The mitogenome is a circular molecule of 16,544 bp in length including the structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region). Within the control region, we identify the termination-associated sequence domain (TAS), central conserved domain (CSB-F, CSB-E and CSB-D), and three conserved sequence blocks (CSB-1, CSB-2 and CSB-3).
采用长PCR和引物步移法测定银鲳的完整线粒体基因组序列。线粒体基因组是一个长度为16544 bp的环状分子，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和2个非编码区（轻链复制起点和控制区）的结构。在控制区内，我们鉴定出终止相关序列结构域（TAS）、中央保守结构域（CSB-F、CSB-E和CSB-D）以及三个保守序列块（CSB-1、CSB-2和CSB-3）。
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