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(鲤形目:鲤科)线粒体基因组的序列鉴定与系统发育分析

Sequence identification and phylogenetic analysis of the mitochondrial genome of (Cypriniformes: Cyprinidae).

作者信息

Zou Yuanchao, Zhang Junying, Zhang Tao, Xie Meng, Wu Tian

机构信息

College of Life Sciences, Neijiang Normal University, Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Neijiang, China.

出版信息

Mitochondrial DNA B Resour. 2018 Apr 1;3(1):414-415. doi: 10.1080/23802359.2018.1456981.

Abstract

is endemic freshwater fish of China and mainly distributed in the middle and upper reaches of the Yangtze River. The complete mitogenome of was first determined by using the next-generation sequencing in present study. The circular mitogenome followed the expected pattern for vertebrates, being 16,603 bp in length. It contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one displacement loop (D-loop) locus and an origin of replication on the light-strand (OL). Most of the genes are encoded on the heavy strand except ND6 and eight tRNA genes. The overall base composition was 30.78% A, 26.09% T, 26.59% C, 16.54% G, with 56.87% AT. Thirteen intergenic spacers and nine gene overlaps exist in the complete mitochondrial genome. Phylogenetic analysis based on the tandem 13 coding protein genes nucleotide sequences with two different methods (maximum likelihood and neighbour-joining analysis) indicated that was grouped in one clade with the same genus. The complete mitogenome of can provide a useful data for the further research in Gobioninae. Meanwhile, it also provides help for biological genetics and classification of species.

摘要

是中国的特有淡水鱼类,主要分布于长江中上游。在本研究中,首次通过下一代测序确定了其完整的线粒体基因组。该环状线粒体基因组遵循脊椎动物的预期模式,长度为16,603 bp。它包含13个蛋白质编码基因、两个核糖体RNA基因、22个转运RNA基因、一个位移环(D-loop)位点和一个轻链复制起点(OL)。除ND6和8个tRNA基因外,大多数基因在重链上编码。总体碱基组成为30.78%A、26.09%T、26.59%C、16.54%G,AT含量为56.87%。完整的线粒体基因组中存在13个基因间隔区和9个基因重叠区。基于串联的13个编码蛋白质基因核苷酸序列,采用两种不同方法(最大似然法和邻接法分析)进行的系统发育分析表明,与同属的物种聚在一个分支中。该物种的完整线粒体基因组可为进一步研究鮈亚科提供有用的数据。同时,也为物种的生物遗传学和分类提供帮助。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a277/7800506/bc2b5d508420/TMDN_A_1456981_F0001_B.jpg

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