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1918年弗里斯线粒体基因组的新一代测序(膜翅目:蜜蜂科)

Next-generation sequencing of the mitochondrial genome of Friese, 1918 (Hymenoptera: Apidae).

作者信息

Zhou Feng, Song Fan, Yao Liyuan, Hou Zhibo, Ning Yingzhi, Chen Lingyun

机构信息

College of Life Science, Northwest Normal University, Lanzhou, China.

Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China.

出版信息

Mitochondrial DNA B Resour. 2021 Sep 24;6(10):3049-3051. doi: 10.1080/23802359.2021.1976691. eCollection 2021.

DOI:10.1080/23802359.2021.1976691
PMID:34589587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8475120/
Abstract

is the species of Bumblebees (Hymenoptera: Apidae), which are important pollinators for wild plants and greenhouses crops. The complete mitochondrial genome (mitogenome) of was determined by next-generation sequencing. The mitogenome was 18,458 bp in size with 87.2% A + T content, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an AT-rich control region (D-loop). Gene arrangement was found to be identical to those of other mitogenomes of bumblebees (e.g. and ). All 13 PCGs initiated with typical ATN codons. Among them, 11 PCGs terminated with TAA or TAG; only and have incomplete stop codon T. All 22 tRNAs can be folded into typical cloverleaf structure. Phylogenetic analysis based on the concatenated nucleotide sequences of all 13 PCGs indicated that was more closely related to other species of subgenus , which clustered into a monophyletic group.

摘要

是熊蜂属(膜翅目:蜜蜂科)的物种,是野生植物和温室作物的重要传粉者。通过下一代测序确定了其完整的线粒体基因组(线粒体基因组)。线粒体基因组大小为18,458 bp,A+T含量为87.2%,包含13个蛋白质编码基因(PCG)、22个tRNA基因、2个rRNA基因和一个富含AT的控制区(D环)。发现基因排列与其他熊蜂线粒体基因组(如和)的基因排列相同。所有13个PCG均以典型的ATN密码子起始。其中,11个PCG以TAA或TAG终止;只有和具有不完整的终止密码子T。所有22个tRNA都可以折叠成典型的三叶草结构。基于所有13个PCG的串联核苷酸序列的系统发育分析表明,与亚属的其他物种关系更密切,它们聚为一个单系类群。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ef/8475120/be3e0e3415ab/TMDN_A_1976691_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ef/8475120/be3e0e3415ab/TMDN_A_1976691_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ef/8475120/be3e0e3415ab/TMDN_A_1976691_F0001_C.jpg

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