Vu Huyen-Trang, Tran Ngan, Nguyen Thanh-Diem, Vu Quoc-Luan, Bui My-Huyen, Le Minh-Tri, Le Ly
Faculty of Biotechnology, Nguyen Tat Thanh University, District 4, Hochiminh City 72820, Vietnam.
Faculty of Biotechnology, International University-Vietnam National University, Linh Trung Ward, Thu Duc District, Hochiminh City 7000000, Vietnam.
Plants (Basel). 2020 Jan 2;9(1):61. doi: 10.3390/plants9010061.
is a native orchid of Vietnam with highly attractive floral traits. Unfortunately, it is now listed as a critically endangered species with a few hundred individuals remaining in nature. In this study, we performed next-generation sequencing of and assembled its complete chloroplast genome. The whole chloroplast genome of was 160,955 bp in size, 35.6% of which was GC content, and exhibited typical quadripartite structure of plastid genomes with four distinct regions, including the large and small single-copy regions and a pair of inverted repeat regions. There were, in total, 130 genes annotated in the genome: 77 coding genes, 39 tRNA genes, 8 rRNA genes, and 6 pseudogenes. The loss of genes and variation in inverted repeat (IR) boundaries as well as data of simple sequence repeats (SSRs) and divergent hotspots provided useful information for identification applications and phylogenetic studies of species. Whole chloroplast genomes could be used as an effective super barcode for species identification or for developing other identification markers, which subsequently serves the conservation of species.
是越南的一种本土兰花，具有极具吸引力的花卉特征。不幸的是，它现在被列为极度濒危物种，自然界仅存几百株。在本研究中，我们对其进行了二代测序并组装出其完整的叶绿体基因组。该植物的叶绿体全基因组大小为160,955 bp，GC含量为35.6%，呈现出典型的质体基因组四分体结构，有四个不同区域，包括大单拷贝区、小单拷贝区和一对反向重复区。基因组中共注释了130个基因：77个编码基因、39个tRNA基因、8个rRNA基因和6个假基因。基因的丢失、反向重复（IR）边界的变异以及简单序列重复（SSR）和分歧热点的数据为该物种的鉴定应用和系统发育研究提供了有用信息。叶绿体全基因组可作为物种鉴定的有效超级条形码或用于开发其他鉴定标记，进而服务于该物种的保护。
