Center for Advanced Technology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614, Poznań, Poland.
Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Mazowiecka 48, 60-623, Poznań, Poland.
Anal Bioanal Chem. 2021 Mar;413(7):1893-1903. doi: 10.1007/s00216-021-03157-2. Epub 2021 Jan 20.
Positional isomers of bisphenol F diglycidyl ether (BFDGE) have been analyzed by high-pressure liquid chromatography-mass spectrometry and by gas chromatography-mass spectrometry (HPLC-MS, GC-MS). Positional isomers of BFDGE derivatives (BFDGEx2HO, BFDGExHOxHCl) have been analyzed by HPLC-MS. On the basis of the obtained fragmentation patterns, the elution order of the isomers has been unequivocally determined, in standard solutions and in the sample of liquid obtained after rinsing an empty mackerel fish can with acetonitrile. Under HPLC condition, para,para isomers are eluted first, then ortho,para isomers' elution follows, and ortho,ortho isomers are eluted last. Under GC condition, the reverse elution order has been obtained. For the first time, two ortho,para isomers of BFDGExHOxHCl have been detected and their elution order has been determined. The obtained results are of key importance for determination of the isomer distribution of BFDGE and its derivatives in food samples.
双酚 F 二缩水甘油醚（BFDGE）的位置异构体已通过高压液相色谱-质谱法和气相色谱-质谱法（HPLC-MS、GC-MS）进行了分析。BFDGE 衍生物（BFDGEx2HO、BFDGExHOxHCl）的位置异构体已通过 HPLC-MS 进行了分析。根据获得的碎片模式，在标准溶液和用乙腈冲洗空鲭鱼罐后获得的液体样品中，明确确定了异构体的洗脱顺序。在 HPLC 条件下，para,para 异构体首先洗脱，然后是 ortho,para 异构体的洗脱，最后是 ortho,ortho 异构体的洗脱。在 GC 条件下，得到了相反的洗脱顺序。首次检测到 BFDGExHOxHCl 的两种 ortho,para 异构体，并确定了它们的洗脱顺序。这些结果对于确定食品样品中 BFDGE 及其衍生物的异构体分布具有重要意义。
