Kizilay Gulnur, Ersoy Onur, Cerkezkayabekir Aysegul, Topcu-Tarladacalisir Yeter
Department of Histology and Embryology, School of Medicine, Trakya University, Edirne, Turkey.
Department of Pathology Laboratory Techniques, Vocational School of Health Services, Trakya University, Edirne, Turkey.
Andrologia. 2021 Apr;53(3):e13858. doi: 10.1111/and.13858. Epub 2021 Jan 21.
Sitagliptin increases the levels of incretin hormones and stimulates a decrease in blood glucose levels, by blocking the DPP4 enzyme. We have very limited information about impact of sitagliptin on male genital system and relationship between sitagliptin/diabetes/ER. Fucoidan can be effective in blood glucose homeostasis. We goal to explain of the effect of sitagliptin and introduce an approach of fucoidan treatment in experimental diabetes in male rats. Fifty-eight Wistar albino rats were divided into C-control group and D-diabetes group: 60 mg/kg streptozotocin intraperitoneal (i.p.); DS group: STZ + 10 mg/kg sitagliptin intragastric (i.g.); DF group: STZ + 100 mg/kg fucoidan i.p.; and DSF group: STZ + 10 mg/kg sitagliptin + 100 mg/kg fucoidan. A significant decrease was detected when DS, DF and DSF groups compared to group D in blood glucose levels, basement membrane thickness and also apoptotic cell/tubule index, pJNK, caspase 3, caspase 12, GRP78, CHOP and DPP4. Sitagliptin and fucoidan have been found to be effective in blood glucose homeostasis and reducing the expression of certain proteins that lead to apoptosis and especially the proteins in the ER stress pathway. Therefore, we think that both sitagliptin and fucoidan can be effective in preventing or eliminating histopathological damages in diabetic testicular tissues, and their treatment effects can be used more.
西他列汀通过抑制二肽基肽酶4（DPP4），提高肠促胰岛素水平，并刺激血糖水平下降。我们对西他列汀对男性生殖系统的影响以及西他列汀/糖尿病/内质网（ER）之间的关系了解非常有限。岩藻依聚糖对血糖稳态可能有效。我们旨在解释西他列汀的作用，并介绍岩藻依聚糖对雄性大鼠实验性糖尿病的治疗方法。58只Wistar白化大鼠分为C对照组和D糖尿病组：腹腔注射（i.p.）60mg/kg链脲佐菌素；DS组：链脲佐菌素+10mg/kg西他列汀灌胃（i.g.）；DF组：链脲佐菌素+100mg/kg岩藻依聚糖腹腔注射；DSF组：链脲佐菌素+10mg/kg西他列汀+100mg/kg岩藻依聚糖。与D组相比，DS、DF和DSF组的血糖水平、基底膜厚度以及凋亡细胞/小管指数、磷酸化JNK、半胱天冬酶3、半胱天冬酶12、葡萄糖调节蛋白78（GRP78）、 Chop和DPP4均显著降低。已发现西他列汀和岩藻依聚糖对血糖稳态有效，并能降低某些导致细胞凋亡的蛋白质尤其是内质网应激途径中蛋白质的表达。因此，我们认为西他列汀和岩藻依聚糖均可有效预防或消除糖尿病睾丸组织的组织病理学损伤，其治疗效果值得更多应用。
