Biomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
Mol Biol Rep. 2021 Feb;48(2):1233-1241. doi: 10.1007/s11033-021-06135-0. Epub 2021 Jan 21.
The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb), connective tissue growth factor (Ctgf), insulin-like growth factor 1 (Igf1), tenascin C (Tnc), Fibronectin (Fn1), Scleraxis (Scx), Fibromodulin (Fmod) and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell migration was analyzed between days 0 and 4 in both fibroblasts (F) and fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene expression were also analyzed after daily MC application. Higher expression of Ctgf and lower expression of Tnc and Fmod, respectively, were observed in the F+MC group in relation to F group (p < 0.05), and no difference was observed between the groups for the genes Tgfb, Fn1 and Scx. In cell migration, a higher number of cells in the scratch region was observed in group F+MC (p < 0.05) compared to group F on the 4th day, and the cell viability assay showed no difference between the groups. In conclusion, MC therapy at an intensity/time of 10 μA/90 s with 4 daily applications did not affect cell viability, stimulated fibroblasts migration with the involvement of Ctgf, and reduced the Tnc and Fmod expression.
文献表明微电流 (MC) 疗法对组织修复具有有益作用。我们研究了以 10 μA/90 s 的电流强度和时间应用 MC 是否可以调节转化生长因子 β (Tgfb)、结缔组织生长因子 (Ctgf)、胰岛素样生长因子 1 (Igf1)、腱糖蛋白 C (Tnc)、纤连蛋白 (Fn1)、肌腱调蛋白 (Scx)、纤维连接蛋白 (Fmod) 和肌腱调蛋白在伤口愈合测定中在 NIH/3T3 成纤维细胞中的表达。在第 0 天和第 4 天之间分析了成纤维细胞 (F) 和成纤维细胞+MC (F+MC) 两组中的细胞迁移。在第 4 天，还分析了每日 MC 应用后细胞活力和基因表达。与 F 组相比，F+MC 组中 Ctgf 的表达更高，而 Tnc 和 Fmod 的表达更低 (p<0.05)，而 Tgfb、Fn1 和 Scx 基因在两组之间没有差异。在细胞迁移方面，与 F 组相比，第 4 天 F+MC 组划痕区域中的细胞数量更多 (p<0.05)，而细胞活力测定显示两组之间没有差异。总之，以 10 μA/90 s 的强度/时间进行 4 次每日 MC 治疗不会影响细胞活力，刺激成纤维细胞迁移并减少 Tnc 和 Fmod 的表达。
