Montpetit M, Abrahams P, Clark A F, Tenniswood M
Department of Biochemistry, University of Ottawa, Ontario, Canada.
Prostate. 1988;12(1):13-28. doi: 10.1002/pros.2990120104.
Androgen-independent cell lines have been clonally selected from primary cultures of androgen-dependent epithelial cells from the rat ventral prostate. These rapidly dividing epithelial-like cells (RDE) have altered morphology and adherence characteristics. Unlike normal prostate epithelial cells, the RDE cell lines do not require androgens for cell division or cell survival. In the presence of physiological concentrations of testosterone, the isoelectric focusing patterns of prostatic acid phosphatases are abnormal in these RDE cells, and the prostate steroid-binding protein genes are not expressed. The loss of androgen dependence is not due to the inability of RDE cells to metabolize testosterone to 5 alpha-dihydrotestosterone, the active androgen, since the RDE cell lines metabolize testosterone in a manner similar to normal androgen-dependent epithelial cells. When RDE cells are grown on collagen matrices, the cells assume ductlike structures, similar to prostatic acini, although PSBP gene expression is not induced. When seeded into soft agar these cell lines form distinct foci, suggesting that they are potentially tumorigenic.
已从大鼠腹侧前列腺雄激素依赖性上皮细胞的原代培养物中克隆选择出雄激素非依赖性细胞系。这些快速分裂的上皮样细胞（RDE）具有改变的形态和黏附特性。与正常前列腺上皮细胞不同，RDE细胞系在细胞分裂或细胞存活方面不需要雄激素。在生理浓度的睾酮存在下，这些RDE细胞中前列腺酸性磷酸酶的等电聚焦模式异常，并且前列腺类固醇结合蛋白基因不表达。雄激素依赖性的丧失并非由于RDE细胞无法将睾酮代谢为活性雄激素5α-二氢睾酮，因为RDE细胞系以与正常雄激素依赖性上皮细胞相似的方式代谢睾酮。当RDE细胞在胶原基质上生长时，细胞呈现出类似于前列腺腺泡的导管样结构，尽管未诱导PSBP基因表达。当接种到软琼脂中时，这些细胞系形成明显的集落，表明它们具有潜在的致瘤性。
