Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
Department of Hematology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
Cancer Lett. 2021 Apr 10;503:54-68. doi: 10.1016/j.canlet.2020.12.039. Epub 2021 Jan 19.
Glioma is the most prevalent intracranial tumour, with considerable morbidity. Long non-coding RNAs are important in the biological processes of various cancers. However, little is known about ST7 antisense RNA 1 (ST7-AS1) and its role in glioma progression. ST7-AS1 expression was reduced in glioma tissues and cells in comparison to normal brain tissues. p53 transcriptionally targeted the ST7-AS1 promoter in U251 glioma cells. The targeting significantly inhibited cell migration, invasion, and proliferation, and promoted apoptosis. ST7-AS1 directly bound to and downregulated polypyrimidine tract-binding protein 1 (PTBP1) at the post-transcriptional level. ST7-AS1 overexpression inhibited glioma progression by suppressing Wnt/β-catenin signalling by downregulating PTBP1 expression. Additionally, p53 expression negatively correlated with PTBP1 expression. Glioma progression is regulated by a positive feedback loop involving the p53/ST7-AS1/PTBP1 axis, which might be a promising therapeutic target for glioma treatment.
神经胶质瘤是最常见的颅内肿瘤，具有相当高的发病率。长链非编码 RNA 在各种癌症的生物学过程中起着重要作用。然而，关于 ST7 反义 RNA 1（ST7-AS1）及其在神经胶质瘤进展中的作用知之甚少。与正常脑组织相比，ST7-AS1 在神经胶质瘤组织和细胞中的表达降低。p53 在 U251 神经胶质瘤细胞中转录靶向 ST7-AS1 启动子。这种靶向作用显著抑制细胞迁移、侵袭和增殖，并促进细胞凋亡。ST7-AS1 直接结合并下调多嘧啶 tract-binding protein 1（PTBP1）在转录后水平。ST7-AS1 通过下调 PTBP1 表达抑制 Wnt/β-catenin 信号通路抑制神经胶质瘤进展。此外，p53 表达与 PTBP1 表达呈负相关。神经胶质瘤的进展受 p53/ST7-AS1/PTBP1 轴的正反馈环调控，这可能是神经胶质瘤治疗的有希望的治疗靶点。
