Department of Computer Science and Engineering, University of Calcutta, West Bengal, India.
Department of Mathematics, Pingla Thana Mahavidyalaya, Maligram, Paschim Medinipur, West Bengal, India.
Infect Genet Evol. 2021 Apr;89:104724. doi: 10.1016/j.meegid.2021.104724. Epub 2021 Jan 19.
Clades are monophyletic groups composed of a common ancestor and all its lineal descendants. As the propensity of virulence of a disease depends upon the type of clade the virus belongs to and it causes different fatality rates of disease in different countries, so the clade-wise analysis of SARS-CoV-2 isolates collected from different countries can illuminate the actual evolutionary relationships between them. In this study, 1566 SARS-CoV-2 genome sequences across ten Asian countries are collected, clustered, and characterized based on the clade they belong to. The isolates are compared to the Wuhan reference sequence" hCoV-19/Wuhan/WIV04/19″ to identify the mutations that occurred at different protein regions. Structural changes in amino acids due to mutations lead to functional instability of the proteins. Detailed clade-wise functional assessments are carried out to quantify the stability and vulnerability of the mutations occurring in SARS-CoV-2 genomes which can shade light on personalized prevention and treatment of the disease and encourage towards the invention of clade-specific vaccines.
进化枝是由一个共同的祖先及其所有直系后代组成的单系群。由于疾病的毒力倾向取决于病毒所属的进化枝类型，并且在不同国家导致不同的疾病致死率，因此对来自不同国家的 SARS-CoV-2 分离株进行进化枝分析可以阐明它们之间的实际进化关系。在这项研究中，收集了来自亚洲十个国家的 1566 个 SARS-CoV-2 基因组序列，根据它们所属的进化枝进行聚类和特征分析。将这些分离株与武汉参考序列“hCoV-19/Wuhan/WIV04/19”进行比较，以确定在不同蛋白区域发生的突变。由于突变导致氨基酸结构变化，从而导致蛋白功能不稳定。对 SARS-CoV-2 基因组中发生的突变进行详细的进化枝功能评估，以量化突变的稳定性和脆弱性，这可以为疾病的个性化预防和治疗提供启示，并鼓励针对特定进化枝的疫苗发明。
