ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, Wollongong, NSW 2500, Australia.
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, Wollongong, NSW 2500, Australia.
Acta Biomater. 2021 Mar 15;123:286-297. doi: 10.1016/j.actbio.2021.01.021. Epub 2021 Jan 18.
Wounds impact millions of patients every year and represent a serious cause of morbidity and mortality worldwide, yet current treatment outcomes are far from ideal. Therapies based on delivery of multiple growth factors offer a promising approach for optimal wound management; however, their high production cost, low stability, and lack of effective delivery system limits their application in the clinic. Platelet lysate is a suitable, abundant and cost-effective source of growth factors that play an important role in the healing cascade. The aim of this current work is to develop an extrusion-based bioink consisting of platelet lysate (PL) and gelatin methacryloyl (GelMA) (PLGMA) for the fabrication of a multifunctional 3D printed dermal equivalent. This bioink meets the essential requirements of printability in terms of rheological properties and shape fidelity. Moreover, its mechanical properties can be readily tuned to achieve stiffness that is equivalent to native skin tissue. Biologically relevant factors were successfully released in a sustainable manner for up to two weeks of study. The bioavailability of those factors was demonstrated by high cell viability, good cell attachment and improved proliferation of printed dermal fibroblasts. Furthermore, growth factors upregulated ECM synthesis and deposition by dermal fibroblasts after two weeks of culture.
伤口每年影响数百万患者，是全球发病率和死亡率的一个严重原因，但目前的治疗效果远不理想。基于多种生长因子传递的治疗方法为优化伤口管理提供了一种有前途的方法；然而，其高生产成本、低稳定性和缺乏有效的传递系统限制了它们在临床上的应用。血小板裂解液是一种合适、丰富且具有成本效益的生长因子来源，在愈合级联反应中发挥着重要作用。本研究旨在开发一种基于挤出的生物墨水，由血小板裂解液（PL）和明胶甲基丙烯酰（GelMA）（PLGMA）组成，用于制造多功能 3D 打印皮肤等效物。该生物墨水在可打印性方面具有流变性能和形状保真度等基本要求。此外，其机械性能可以很容易地进行调整，以达到与天然皮肤组织相当的刚度。在长达两周的研究中，生物相关因子以可持续的方式成功释放。通过高细胞活力、良好的细胞附着和提高打印皮肤成纤维细胞的增殖，证明了这些因子的生物利用度。此外，生长因子在培养两周后上调了皮肤成纤维细胞的 ECM 合成和沉积。
