College of Life Sciences, Sichuan University, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, China.
College of Life Sciences, Sichuan University, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, China.
J Glob Antimicrob Resist. 2021 Mar;24:266-269. doi: 10.1016/j.jgar.2020.12.023. Epub 2021 Jan 18.
The global spread of the New Delhi metallo-β-lactamase 1 (NDM-1) gene poses a significant challenge to worldwide public health. Here, we characterize the whole genome of NDM-1-producing Proteus mirabilis isolate SNYG35 of broiler chicken origin in China.
The genome of SNYG35 was sequenced using a PacBio RS II sequencing instrument and Illumina HiSeq platform. SMRT cell data were assembled independently using HGAP4 and Canu v1.6, and were further polished with Illumina data using Pilon v1.22. The presence of antimicrobial resistance genes was identified using CGE ResFinder 3.2. A conjugation experiment was performed using the sodium azide-resistant Escherichia coli J53Azi strain as the recipient.
The chromosome of SNYG35 is 4 014 504 bp in size and consists of one chromosome and one plasmid named pSNYG35. It contains 3646 coding sequences, 82 tRNA genes, 22 rRNAs, and four non-coding RNAs. Besides bla, SNYG35 harbours 26 different antimicrobial resistance genes including ESBL gene bla as well as fluoroquinolone and aminoglycoside resistance gene aac(6')-Ib-cr. The bla-harbouring pSNYG35 is a pPrY2001-like plasmid and shares highest nucleotide identity to pHFK418-NDM. It carries a Tn1696-like multidrug-resistant region harbouring 12 different antimicrobial resistance genes, and could be transferred to E. coli J53.
Here, we report for the first time the whole genome sequence of a NDM-1-producing P. mirabilis isolate from broiler chicken in China, which provides valuable information for tracing the potential transmission of NDM-1-producing P. mirabilis from broiler chicken to humans, as well as revealing the spread and evolution of bla-harbouring pPrY2001-like plasmids.
新德里金属β-内酰胺酶 1（NDM-1）基因在全球范围内的传播对全球公共卫生构成了重大挑战。在这里，我们对中国起源于肉鸡的产 NDM-1 奇异变形杆菌 SNYG35 进行了全基因组特征描述。
使用 PacBio RS II 测序仪和 Illumina HiSeq 平台对 SNYG35 的基因组进行测序。SMRT 细胞数据分别使用 HGAP4 和 Canu v1.6 进行组装，并使用 Pilon v1.22 结合 Illumina 数据进行进一步的抛光处理。使用 CGE ResFinder 3.2 识别抗生素耐药基因的存在。通过使用耐叠氮化钠的大肠杆菌 J53Azi 菌株作为受体进行了接合实验。
SNYG35 的染色体大小为 4014504 bp，由一条染色体和一个名为 pSNYG35 的质粒组成。它包含 3646 个编码序列、82 个 tRNA 基因、22 个 rRNA 和 4 个非编码 RNA。除了 bla 基因外，SNYG35 还含有 26 种不同的抗生素耐药基因，包括 ESBL 基因 bla 以及氟喹诺酮和氨基糖苷类耐药基因 aac(6')-Ib-cr。携带 bla 基因的 pSNYG35 是一种 pPrY2001 样质粒，与 pHFK418-NDM 的核苷酸同源性最高。它携带一个 Tn1696 样多药耐药区，含有 12 种不同的抗生素耐药基因，可转移至大肠杆菌 J53。
在这里，我们首次报道了中国肉鸡来源的产 NDM-1 奇异变形杆菌分离株的全基因组序列，为追踪产 NDM-1 奇异变形杆菌从肉鸡向人类的潜在传播提供了有价值的信息，并揭示了 bla 基因携带的 pPrY2001 样质粒的传播和进化。
