Lv Penghao, Hao Guijuan, Cao Yanli, Cui Lulu, Wang Guisheng, Sun Shuhong
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Shandong Animal Disease Prevention and Control Center, Taian 261500, China.
Biology (Basel). 2022 Feb 11;11(2):292. doi: 10.3390/biology11020292.
, an opportunistic pathogen, is found to be an emerging threat to both animals and humans for a variety of infections. However, the characteristics of infections from foxes, raccoons and minks remain unclear. In this context, we identified the antibiotic resistance genes and virulence genes of isolates from foxes, raccoons and minks in China. Most isolates showed resistance to florfenicol (90.57%), trimethoprim-sulfamethoxazole (73.58%), and imipenem (71.70%). A total of 73.58% of isolates were resistant to antibiotics from at least three or more classes, and were categorized as multi-drug resistant. A total of 33.33% of the isolates were resistant to antibiotics from seven classes. The most prevalent resistant were (94.34%), followed by , , and with the detection rate of 88.68%, 83.02%, 71.70% and 60.38%, respectively. Among the 51 isolates that were resistant to beta-lactam antibiotics, all isolates carried at least one beta-lactam gene. In addition, and genes were firstly reported in carbapenem-resistant isolates from foxes, raccoons and minks. All isolates exhibited the virulence genes , , , and . isolates carrying all detected 10 virulence genes from different animal species showed different lethal abilities in a larvae model More importantly, the profiles of antibiotic resistance genes of isolates from fur animals and the environment were generally similar, and phylogenetic analysis showed that the isolates from farm environment samples may have close relatedness with that from animals.
作为一种机会性病原体，已被发现对动物和人类都构成了多种感染的新威胁。然而，狐狸、浣熊和水貂感染的特征仍不清楚。在此背景下，我们鉴定了中国狐狸、浣熊和水貂分离株的抗生素抗性基因和毒力基因。大多数分离株对氟苯尼考（90.57%）、甲氧苄啶-磺胺甲恶唑（73.58%）和亚胺培南（71.70%）耐药。共有73.58%的分离株对至少三类或更多类抗生素耐药，被归类为多重耐药。共有33.33%的分离株对七类抗生素耐药。最常见的耐药菌是[具体菌名]（94.34%），其次是[其他菌名]、[其他菌名]、[其他菌名]和[其他菌名]，检出率分别为88.68%、83.02%、71.70%和60.38%。在51株对β-内酰胺类抗生素耐药的[菌名]分离株中，所有分离株都携带至少一个β-内酰胺基因。此外，[具体基因名]和[具体基因名]基因首次在狐狸、浣熊和水貂的耐碳青霉烯类[菌名]分离株中报道。所有分离株都显示出毒力基因[具体基因名]、[具体基因名]、[具体基因名]、[具体基因名]和[具体基因名]。携带来自不同动物物种的所有10个检测到的毒力基因的[菌名]分离株在[幼虫模型名称]幼虫模型中表现出不同的致死能力。更重要的是，毛皮动物和环境分离株的抗生素抗性基因谱总体相似，系统发育分析表明，来自农场环境样本的[菌名]分离株可能与来自动物的分离株密切相关。
