Department of Neurosurgery, Hacettepe University Faculty of Medicine, Ankara, Turkey.
Department of Histology and Embryology, Hacettepe University Faculty of Medicine, Ankara, Turkey.
J Neuroimmunol. 2021 Mar 15;352:577476. doi: 10.1016/j.jneuroim.2021.577476. Epub 2021 Jan 9.
The receptor for advanced glycation end products (RAGE)- Nuclear Factor kappa B (NF-κB) signal pathway may represent a new target for the treatment of traumatic brain injury (TBI). The aim of the study is to investigate effects of papaverine on secondary signaling mechanisms through this pathway in mice TBI model.Immunohistochemically, while the number of RAGE and NF- κB positive cells, apoptotic cells increased, the number of NeuN positive cells reduced in TBI.Papaverine reduced the number of RAGE positive cells on glia and the number of NF- κB positive cells on both neuron and glia. At the same time, it decreased the number of microglia labeled with P2RY12 increased due to TBI. It also increased the NeuN positive cells and mitigated the brain edema. Results of this study showed that papaverine reduced TBI- induced neuroinflammation and apoptosis, also provided neuroprotection via the RAGE- NF-κB signal path, which is one of the possible mechanisms in TBI.
晚期糖基化终产物（RAGE）-核因子κB（NF-κB）信号通路可能代表创伤性脑损伤（TBI）治疗的新靶点。本研究旨在探讨罂粟碱通过该通路对小鼠 TBI 模型中二次信号机制的影响。免疫组化结果显示，TBI 后 RAGE 和 NF-κB 阳性细胞数增加，神经元核抗原（NeuN）阳性细胞数减少。罂粟碱减少了小胶质细胞上的 RAGE 阳性细胞和神经元及小胶质细胞上的 NF-κB 阳性细胞数。同时，它减少了由于 TBI 而增加的 P2RY12 标记的小胶质细胞数量。它还增加了 NeuN 阳性细胞，减轻了脑水肿。本研究结果表明，罂粟碱通过 RAGE-NF-κB 信号通路减轻 TBI 诱导的神经炎症和细胞凋亡，从而提供神经保护作用，这可能是 TBI 的机制之一。
