Handoo Zafar A, Yan Guiping, Kantor Mihail R, Huang Danqiong, Chowdhury Intiaz A, Plaisance Addison, Bauchan Gary R, Mowery Joseph D
Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705, USA.
Department of Plant Pathology, North Dakota State University, Fargo, ND 58108, USA.
Plants (Basel). 2021 Jan 17;10(1):168. doi: 10.3390/plants10010168.
Root-lesion nematodes ( spp.) of the genus Filipjev, 1936, are among the most important nematode pests on soybean ( (L.) Merr.), along with soybean cyst and root-knot nematodes. In May 2015 and 2016, a total of six soil samples were collected from a soybean field in Walcott, Richland County, ND and submitted to the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), USDA, ARS, MD for analysis. Later, in 2019, additional nematodes recovered from a greenhouse culture on soybean originally from the same field were submitted for further analysis. Males, females, and juveniles of sp. were recovered from soil and root samples and were examined morphologically and molecularly. DNA from single nematodes were extracted, and the nucleotides feature of three genomic regions targeting on the D2-D3 region of 28S rDNA and ITS rDNA and mitochondrial cytochrome oxidase subunit I () gene were characterized. Phylogeny trees were constructed to ascertain the relationships with other spp., and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to provide a rapid and reliable differentiation from other common spp. Molecular features indicated that it is a new, unnamed sp. that is different from morphologically closely related spp., including . , , , and . . In conclusion, both morphological and molecular observations indicate that the North Dakota isolate on soybean represents a new root-lesion nematode species which is named and described herein as n. sp.
1936年菲利耶夫属的根病变线虫（spp.）是大豆（(L.) Merr.）上最重要的线虫害虫之一，与大豆孢囊线虫和根结线虫一样。2015年5月和2016年，从北达科他州里奇兰县沃尔科特的一块大豆田总共采集了6份土壤样本，并提交给美国农业部农业研究局位于马里兰州的真菌学和线虫学遗传多样性与生物学实验室（MNGDBL）进行分析。后来，在2019年，从最初来自同一田地的大豆温室培养物中回收的额外线虫被提交进行进一步分析。从土壤和根样本中回收了sp.的雄虫、雌虫和幼虫，并进行了形态学和分子学检查。提取单个线虫的DNA，并对靶向28S rDNA的D2 - D3区域、ITS rDNA和线粒体细胞色素氧化酶亚基I（）基因的三个基因组区域的核苷酸特征进行了表征。构建系统发育树以确定与其他spp.的关系，并进行聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）分析以提供与其他常见spp.的快速可靠区分。分子特征表明它是一种新的、未命名的sp.，与形态学上密切相关的spp.不同，包括.、.、.和..。总之，形态学和分子观察均表明北达科他州大豆上的分离物代表一种新的根病变线虫物种，本文将其命名并描述为n. sp.
