Laboratory of Antioxidants, Nutrition and Food Technology Institute, University of Chile, Santiago 7830490, Chile.
Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago 7830490, Chile.
Molecules. 2021 Jan 17;26(2):463. doi: 10.3390/molecules26020463.
A clear gap with respect to the potential biological properties of wheat flavonoids exists in the available literature. This information is crucial for breeding programs aiming to produce new varieties presenting improved health benefits. Accordingly, advanced breeding lines of whole durum wheat were evaluated in this contribution. The highest recovery of phenolics was achieved using aqueous acetone (50:50, ), as verified by multi-response optimization, thus showing that phenolics could be largely underestimated by employing an inappropriate extraction. The concentration of derivatives of apigenin, the main phenolics present, ranged from 63.5 to 80.7%, as evaluated by LC-ESI-QTOF-MS. Phenolics from the breeding line 98 exhibited the highest ability in scavenging peroxyl radicals, reducing power as well as in terms of inhibition of pancreatic lipase activity, a key enzyme regulating the absorption of triacylglycerols. In contrast, none of the samples exhibited a significant anti-diabetic potential. Despite their high concentration compared to that of phenolic acids, results of this work do not support a significant antioxidant and pancreatic lipase inhibitory effect of durum wheat flavonoids. Therefore, breeding programs and animal and/or human trials related to the effect of durum wheat flavonoids on oxidative stress and absorption of triacylglycerols are discouraged at this point.
关于小麦类黄酮的潜在生物学特性，现有文献中存在明显的空白。这些信息对于旨在生产具有更好健康益处的新品种的育种计划至关重要。因此，本研究评估了全硬质小麦的先进育种品系。通过多响应优化验证，水丙酮（50:50，v/v）的提取效果最好，可实现最大程度的酚类物质回收，表明采用不适当的提取方法可能会大大低估酚类物质的含量。采用 LC-ESI-QTOF-MS 分析，评估了主要类黄酮芹黄素的衍生物的浓度，范围为 63.5%至 80.7%。98 号育种品系的酚类物质具有最强的清除过氧自由基、还原能力以及抑制胰腺脂肪酶活性的能力，胰腺脂肪酶是调节三酰基甘油吸收的关键酶。相比之下，没有一种样品表现出显著的抗糖尿病潜力。尽管与酚酸相比，类黄酮的浓度较高，但本研究的结果并不支持硬质小麦类黄酮具有显著的抗氧化和胰腺脂肪酶抑制作用。因此，目前不鼓励开展与硬质小麦类黄酮对氧化应激和三酰基甘油吸收影响相关的育种计划以及动物和/或人体试验。
