LIMA Laboratory, Faculty of Medicine of Monastir, Monastir University, Av. Avicenne, Monastir 5019, Tunisia.
SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France.
Sensors (Basel). 2021 Jan 17;21(2):619. doi: 10.3390/s21020619.
Several studies were devoted to the design of molecularly imprinted polymer (MIP)-based sensors for the detection of a given protein. Here, we bring elements that could contribute to the understanding of the interaction mechanism involved in the recognition of a protein by an imprint. For this purpose, a polydopamine (PDA)-MIP was designed for bovine serum albumin (BSA) recognition. Prior to BSA grafting, the gold surfaces were functionalized with mixed self-assembled monolayers of (MUDA)/(MHOH) (1/9, ). The MIP was then elaborated by dopamine electropolymerization and further extraction of BSA templates by incubating the electrode in proteinase K solution. Three complementary techniques, electrochemistry, zetametry, and Fourier-transform infrared spectrometry, were used to investigate pH and ionic strength effects on a MIP's design and the further recognition process of the analytes by the imprints. Several MIPs were thus designed in acidic, neutral, and basic media and at various ionic strength values. Results indicate that the most appropriate conditions, to achieve a successful MIPs, were an ionic strength of 167 mM and a pH of 7.4. Sensitivity and dissociation constant of the designed sensor were of order of (3.36 ± 0.13) µA·cm·mg·mL and (8.56 ± 6.09) × 10 mg/mL, respectively.
已有多项研究致力于设计基于分子印迹聚合物（MIP）的传感器，以检测特定蛋白质。在此，我们引入了一些元素，有助于理解印迹识别蛋白质过程中涉及的相互作用机制。为此，设计了一种用于牛血清白蛋白（BSA）识别的聚多巴胺（PDA）-MIP。在接枝 BSA 之前，金表面通过（MUDA）/（MHOH）（1/9，）混合自组装单层进行功能化。然后通过多巴胺电化学聚合进一步提取 BSA 模板，将电极孵育在蛋白酶 K 溶液中。电化学、zetametry 和傅里叶变换红外光谱三种互补技术用于研究 pH 值和离子强度对 MIP 设计以及印迹物对分析物进一步识别过程的影响。因此，在酸性、中性和碱性介质以及不同离子强度值下设计了几种 MIP。结果表明，为了成功制备 MIP，最合适的条件是离子强度为 167 mM，pH 值为 7.4。设计传感器的灵敏度和离解常数分别为（3.36 ± 0.13）µA·cm·mg·mL 和（8.56 ± 6.09）×10 mg/mL。
