Unità di Medicina di Laboratorio e Biotecnologie Avanzate, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Via E. Tricomi 5, 90127 Palermo, Italy.
Unità Prodotti Cellulari (GMP), Fondazione Ri.MED c/o IRCCS-ISMETT, Via E. Tricomi 5, 90127 Palermo, Italy.
Int J Environ Res Public Health. 2021 Jan 18;18(2):779. doi: 10.3390/ijerph18020779.
To evaluate and validate the efficacy of disinfectants used in our cleaning procedure, in order to reduce pharmaceutical hospital surfaces' contaminations, we tested the action of three commercial disinfectants on small representative samples of the surfaces present in our hospital cleanrooms. These samples (or coupons) were contaminated with selected microorganisms for the validation of the disinfectants. The coupons were sampled before and after disinfection and the microbial load was assessed to calculate the Log reduction index. Subsequently, we developed and validated a disinfection procedure on real surfaces inside the cleanrooms intentionally contaminated with microorganisms, using approximately 10-10 total colony forming units per coupon. Our results showed a bactericidal, fungicidal, and sporicidal efficacy coherent to the acceptance criteria suggested by United States Pharmacopeia 35 <1072>. The correct implementation of our cleaning and disinfection procedure, respecting stipulated concentrations and contact times, led to a reduction of at least 6 Log for all microorganisms used. The proposed disinfection procedure reduced the pharmaceutical hospital surfaces' contaminations, limited the propagation of microorganisms in points adjacent to the disinfected area, and ensured high disinfection and safety levels for operators, patients, and treated surfaces.
为了评估和验证我们清洁程序中使用的消毒剂的功效，以减少医院医药表面的污染，我们测试了三种商业消毒剂对我们医院洁净室中存在的代表性小表面样本的作用。这些样本（或优惠券）被选定的微生物污染，以验证消毒剂的效果。在消毒前后对优惠券进行采样，并评估微生物负荷以计算对数减少指数。随后，我们在洁净室内的实际表面上开发并验证了一种消毒程序，这些表面故意被微生物污染，每个优惠券使用大约 10-10 个总菌落形成单位。我们的结果显示，杀菌、杀菌和杀孢子效果与美国药典 35<1072>建议的验收标准一致。正确实施我们的清洁和消毒程序，遵守规定的浓度和接触时间，可使所有使用的微生物减少至少 6 对数。所提出的消毒程序减少了医药医院表面的污染，限制了消毒区域附近微生物的繁殖，并确保了操作人员、患者和处理表面的高消毒和安全水平。
