Tyski Stefan, Grzybowska Wanda, Bocian Ewa
Department of Antibiotics and Microbiology, National Medicines Institute, 00-725 Warsaw, Poland.
Department of Pharmaceutical Microbiology, Medical University of Warsaw, 02-007 Warsaw, Poland.
Int J Environ Res Public Health. 2021 May 31;18(11):5932. doi: 10.3390/ijerph18115932.
The purpose of disinfectants is to reduce microorganisms on a contaminated surface and to prevent the spread of microorganisms. The relatively new EN 16615 simulates disinfection by wiping and allows for assessing the recovery of microorganisms from the surface and, importantly, the degree of spread of microorganisms when the surface is disinfected by wiping. For the first time, using this standard, the tested products in the form of commercial disinfectant wipes were compared with self-made wipes soaked in respective disinfectant liquids. The disinfected surfaces were simulated by homogeneous polyvinyl chloride plates. The studies were carried out not only with the standard, but also with clinical multidrug-resistant microbial strains. Based on the research, it can be concluded that the most effective products in the disinfection process (log reduction of ≥5) with the shortest contact time (1 min) were products containing ethanol, propanol, and quaternary ammonium compounds (self-made wipes) and propanol (commercial wipes). The least effective products (log reduction of <5) in terms of the contact time declared by the manufacturer were products containing ethanol and sodium hypochlorite (commercial wipes). Much better antimicrobial activity of self-made wipes was observed in comparison to the activity of the commercial wipes.
消毒剂的目的是减少污染表面的微生物并防止微生物传播。相对较新的EN 16615标准模拟擦拭消毒，并允许评估从表面回收微生物的情况，重要的是，还能评估通过擦拭对表面进行消毒时微生物的传播程度。首次使用该标准，将市售消毒湿巾形式的受试产品与浸泡在各自消毒液体中的自制湿巾进行了比较。用均质聚氯乙烯板模拟消毒表面。研究不仅按照该标准进行，还使用了临床多重耐药微生物菌株。基于这些研究，可以得出结论，在消毒过程中（对数减少≥5）且接触时间最短（1分钟）的最有效产品是含有乙醇、丙醇和季铵化合物的产品（自制湿巾）以及丙醇（市售湿巾）。就制造商宣称的接触时间而言，最无效的产品（对数减少<5）是含有乙醇和次氯酸钠的产品（市售湿巾）。与市售湿巾相比，自制湿巾的抗菌活性要好得多。
