Telagathoti Anusha, Probst Maraike, Khomenko Iuliia, Biasioli Franco, Peintner Ursula
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Fondazione Edmund Mach, Research and Innovation Centre, Food Quality and Nutrition Department, Via Edmund Mach 1, 38010 San Michele all'Adige, Italy.
J Fungi (Basel). 2021 Jan 19;7(1):66. doi: 10.3390/jof7010066.
In ecology, Volatile Organic Compounds (VOCs) have a high bioactive and signaling potential. VOCs are not only metabolic products, but are also relevant in microbial cross talk and plant interaction. Here, we report the first large-scale VOC study of 13 different species of ) isolated from a range of different alpine environments. Proton Transfer Reaction-Time-of-Flight Mass Spectrometry (PTR-ToF-MS) was applied for a rapid, high-throughput and non-invasive VOC fingerprinting of 72 isolates growing under standardized conditions. Overall, we detected 139 mass peaks in the headspaces of all 13 species studied here. Thus, species generally produce a high number of different VOCs. species could clearly be discriminated based on their volatilomes, even if only high-concentration mass peaks were considered. The volatilomes were partially phylogenetically conserved. There were no VOCs produced by only one species, but the relative concentrations of VOCs differed between species. From a univariate perspective, we detected mass peaks with distinctively high concentrations in single species. Here, we provide initial evidence that VOCs may provide a competitive advantage and modulate species distribution on a global scale.
在生态学中，挥发性有机化合物（VOCs）具有很高的生物活性和信号传导潜力。VOCs不仅是代谢产物，还与微生物间相互作用和植物相互作用相关。在此，我们报告了对从一系列不同高山环境中分离出的13种不同（此处原文缺失相关物种信息）物种进行的首次大规模VOC研究。采用质子转移反应-飞行时间质谱法（PTR-ToF-MS）对在标准化条件下生长的72株（此处原文缺失相关菌株信息）分离株进行快速、高通量且非侵入性的VOC指纹图谱分析。总体而言，我们在此研究的所有13种（此处原文缺失相关物种信息）物种的顶空中检测到139个质量峰。因此，（此处原文缺失相关物种信息）物种通常会产生大量不同的VOCs。即使仅考虑高浓度质量峰，（此处原文缺失相关物种信息）物种也可根据其挥发物组明显区分开来。挥发物组在系统发育上部分保守。不存在仅由一个物种产生的VOCs，但不同物种之间VOCs的相对浓度有所不同。从单变量角度来看，我们在单个物种中检测到浓度明显较高的质量峰。在此，我们提供了初步证据表明VOCs可能在全球范围内提供竞争优势并调节（此处原文缺失相关物种信息）物种分布。
