Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), C/Serrano 115 dpdo, 28006 Madrid, Spain.
Viruses. 2021 Jan 19;13(1):137. doi: 10.3390/v13010137.
The green peach aphid Sulzer is the main vector of the semipersistently transmitted and phloem-limited (BYV, ). Studies monitoring the probing behavior by using the Electrical penetration graphs (EPG) technique revealed that inoculation of BYV occurs during unique brief intracellular punctures (phloem-pds) produced in companion and/or sieve element cells. Intracellular stylet punctures (or pds) are subdivided in three subphases (II-1, II-2 and II-3), which have been related to the delivery or uptake of non-phloem limited viruses transmitted in a non-persistent or semipersistent manner. As opposed to non-phloem limited viruses, the specific pd subphase(s) involved in the successful delivery of phloem limited viruses by aphids remain unknown. Therefore, we monitored the feeding process of BYV-carrying individuals in sugar beet plants by the EPG technique and the feeding process was artificially terminated at each phloem-pd subphase. Results revealed that aphids that only performed the subphase II-1 of the phloem-pd transmitted BYV at similar efficiency than those allowed to perform subphase II-2 or the complete phloem-pd. This result suggests that BYV inoculation occurs during the first subphase of the phloem-pd. The specific transmission mechanisms involved in BYV delivery in phloem cells are discussed.
绿桃蚜 （Sulzer） 是半持久型和韧皮部限制型（BYV）的主要载体。使用电穿透图（EPG）技术监测探测行为的研究表明，接种 BYV 发生在伴胞和/或筛管细胞中产生的独特短暂细胞内穿孔（韧皮部-pds）期间。细胞内刺探（或 pds）被细分为三个亚相位（II-1、II-2 和 II-3），这些亚相位与以非持久型或半持久型传播的非韧皮部限制型病毒的传递或摄取有关。与非韧皮部限制型病毒相反，蚜虫成功传递韧皮部限制型病毒所涉及的特定 pd 亚相位尚不清楚。因此，我们通过 EPG 技术监测携带 BYV 的个体在糖甜菜植物中的取食过程，并在每个韧皮部-pd 亚相位人工终止取食过程。结果表明，仅执行韧皮部-pd 的亚相位 II-1 的蚜虫以与允许执行亚相位 II-2 或完整韧皮部-pd 的蚜虫相似的效率传播 BYV。这一结果表明，BYV 接种发生在韧皮部-pd 的第一个亚相位期间。讨论了涉及 BYV 在韧皮部细胞中传递的特定传播机制。
