Departamento de Protección Vegetal, Instituto de Ciencias Agrarias, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain.
EPG systems, Dillenburg 12, 6703 CJ Wageningen, The Netherlands.
J Gen Virol. 2012 Mar;93(Pt 3):662-667. doi: 10.1099/vir.0.037887-0. Epub 2011 Nov 16.
Inoculation of the semi-persistent cauliflower mosaic virus (CaMV, genus Caulimovirus) is associated with successive brief (5-10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. In contrast to non-persistent viruses, there is no evidence for which of the pd subphases (II-1, II-2 and II-3) is involved in the inoculation of CaMV. Experiments were conducted using the electrical penetration graph (EPG) technique to investigate which particular subphases of the pd are associated with the inoculation of CaMV to turnip by its aphid vector Brevicoryne brassicae. In addition, the same aphid species/test plant combination was used to compare the role of the pd subphases in the inoculation of the non-persistent turnip mosaic virus (TuMV, genus Potyvirus). Inoculation of TuMV was found to be related to subphase II-1, confirming earlier results, but CaMV inoculation appeared to be related exclusively to subphase II-2 instead. The mechanism of CaMV inoculation and the possible nature of subphase II-2 are discussed in the scope of our findings.
接种半持久型花椰菜花叶病毒（CaMV，Caulimovirus 属）时，蚜虫在探测表皮和叶肉细胞时会进行连续短暂（5-10 秒）的细胞内刺吸（pd）。与非持久型病毒不同，没有证据表明 pd 的哪个亚期（II-1、II-2 和 II-3）参与了 CaMV 的接种。本实验使用电穿透图（EPG）技术，研究了 Brevicoryne brassicae 蚜虫将 CaMV 接种到萝卜中的 pd 的特定亚期。此外，还使用相同的蚜虫物种/测试植物组合来比较 pd 亚期在接种非持久型萝卜花叶病毒（TuMV，Potyvirus 属）中的作用。结果发现，接种 TuMV 与亚期 II-1 有关，这证实了早期的结果，但 CaMV 的接种似乎仅与亚期 II-2 有关。我们的研究结果讨论了 CaMV 接种的机制和亚期 II-2 的可能性质。
