Kumar Awanish, Bisht Meena, Venkatesu Pannuru
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Department of Chemistry, University of Delhi, Delhi, 110 007, India.
Int J Biol Macromol. 2017 Mar;96:611-651. doi: 10.1016/j.ijbiomac.2016.12.005. Epub 2016 Dec 7.
Over the past years since the discovery of ionic liquids (ILs), there is an increased demand to consider ILs as novel biocompatible co-solvents for proteins. Due to their tunable physical properties ILs can adjust themselves in any required experimental conditions starting from protein extraction to enzyme catalysis at elevated temperature. In recent years, large numbers of ILs have been synthesized and their effect on protein stability has been illustrated. With the rapid growth in various kinds of ILs, our understanding of protein stability in ILs has substantially increased. It is not necessary that a particular IL that is biocompatible to a protein will behave same for the other. Therefore, it is extremely essential to collect the literature dealing with the direct involvement of ILs in protein folding/unfolding studies under the same roof. This review focuses the tremendous accomplishments achieved in recent years in the field of protein stability in ILs. We hope that this would also help to set a stage where we can identify, explore and compare the mechanistic behavior of protein folding/unfolding in ILs. This review will surely bring a new boost in protein folding studies from the chemical biology perspective.
自离子液体（ILs）被发现以来的过去几年里，将离子液体视为蛋白质新型生物相容性共溶剂的需求日益增加。由于其可调节的物理性质，离子液体能够在从蛋白质提取到高温下酶催化的任何所需实验条件下进行自我调节。近年来，大量的离子液体已被合成，并且它们对蛋白质稳定性的影响也已得到阐明。随着各类离子液体的快速增长，我们对蛋白质在离子液体中的稳定性的理解有了显著提高。对一种蛋白质具有生物相容性的特定离子液体，对另一种蛋白质未必也有同样的表现。因此，将涉及离子液体直接参与蛋白质折叠/去折叠研究的文献集中起来是极其必要的。本综述聚焦于近年来在离子液体中蛋白质稳定性领域所取得的巨大成就。我们希望这也将有助于搭建一个平台，在这个平台上我们能够识别、探索并比较蛋白质在离子液体中折叠/去折叠的机制行为。本综述必将从化学生物学的角度为蛋白质折叠研究带来新的推动。
