Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
Open Targets, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
Genome Biol. 2021 Jan 21;22(1):40. doi: 10.1186/s13059-021-02268-4.
CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.
CRISPR 引导 RNA 文库已经被反复改进，以提供越来越高效的试剂，尽管它们的大规模是许多应用的障碍。我们通过挖掘现有的大规模基因功能丧失数据集来设计优化的最小全基因组人类 CRISPR-Cas9 文库（MinLibCas9），与其他 CRISPR-Cas9 文库相比，文库大小减少了 42%以上，同时保持了检测的灵敏度和特异性。MinLibCas9 与现有的数据集具有向后兼容性，增加了 CRISPR-Cas9 筛选的动态范围，并将其应用扩展到复杂的模型和检测中。
