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A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material.

作者信息

Rauch Jennifer N, Valois Eric, Solley Sabrina C, Braig Friederike, Lach Ryan S, Audouard Morgane, Ponce-Rojas Jose Carlos, Costello Michael S, Baxter Naomi J, Kosik Kenneth S, Arias Carolina, Acosta-Alvear Diego, Wilson Maxwell Z

机构信息

University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.

Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, California, USA.

出版信息

J Clin Microbiol. 2021 Mar 19;59(4). doi: 10.1128/JCM.02402-20.

DOI:10.1128/JCM.02402-20
PMID:33478979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8092748/
Abstract

The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/841132629ae3/JCM.02402-20-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/44140ebf046b/JCM.02402-20-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/f5443384eed0/JCM.02402-20-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/fe225e0c31e6/JCM.02402-20-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/531a265e4218/JCM.02402-20-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/841132629ae3/JCM.02402-20-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/44140ebf046b/JCM.02402-20-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/f5443384eed0/JCM.02402-20-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/fe225e0c31e6/JCM.02402-20-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/531a265e4218/JCM.02402-20-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/8092748/841132629ae3/JCM.02402-20-f0005.jpg

相似文献

[1]
A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material.

J Clin Microbiol. 2021-3-19

[2]
CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples.

Curr Protoc. 2022-2

[3]
Comparison of Severe Acute Respiratory Syndrome Coronavirus 2 Screening Using Reverse Transcriptase-Quantitative Polymerase Chain Reaction or CRISPR-Based Assays in Asymptomatic College Students.

JAMA Netw Open. 2021-2-1

[4]
Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection.

PLoS One. 2020-8-13

[5]
Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms.

Drug Discov Today. 2021-8

[6]
CRISPR-based point-of-care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS-CoV-2 detection.

J Biochem Mol Toxicol. 2022-8

[7]
iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2.

Virus Res. 2020-8-18

[8]
Effectiveness and cost-effectiveness of four different strategies for SARS-CoV-2 surveillance in the general population (CoV-Surv Study): a structured summary of a study protocol for a cluster-randomised, two-factorial controlled trial.

Trials. 2021-1-8

[9]
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance.

CRISPR J. 2023-4

[10]
Clinical Performance of Direct RT-PCR Testing of Raw Saliva for Detection of SARS-CoV-2 in Symptomatic and Asymptomatic Individuals.

Microbiol Spectr. 2022-12-21

引用本文的文献

[1]
CRISPR/Cas13-Based Anti-RNA Viral Approaches.

Genes (Basel). 2025-7-25

[2]
CRISPR-Cas Systems Associated with Electrolyte-Gated Graphene-Based Transistors: How They Work and How to Combine Them.

Biosensors (Basel). 2024-11-7

[3]
Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges.

Biosensors (Basel). 2024-9-26

[4]
Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects.

RSC Adv. 2024-10-14

[5]
New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity.

Nucleic Acids Res. 2024-1-25

[6]
CRISPR use in diagnosis and therapy for COVID-19.

Methods Microbiol. 2022

[7]
CRISPR-Cas Technology as a Revolutionary Genome Editing tool: Mechanisms and Biomedical Applications.

Iran Biomed J. 2023-9-1

[8]
CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis.

Int J Mol Sci. 2023-8-30

[9]
Microbial applications for sustainable space exploration beyond low Earth orbit.

NPJ Microgravity. 2023-6-21

[10]
CRISPR/Cas13a-Based MicroRNA Detection in Tumor-Derived Extracellular Vesicles.

Adv Sci (Weinh). 2023-8

本文引用的文献

[1]
A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.

J Clin Microbiol. 2021-3-19

[2]
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay.

Nat Commun. 2020-9-18

[3]
A 3D-printed nasopharyngeal swab for COVID-19 diagnostic testing.

3D Print Med. 2020-8-15

[4]
US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2.

Emerg Infect Dis. 2020-5-15

[5]
CRISPR-Cas12-based detection of SARS-CoV-2.

Nat Biotechnol. 2020-4-16

[6]
Temporal dynamics in viral shedding and transmissibility of COVID-19.

Nat Med. 2020-4-15

[7]
Projecting the transmission dynamics of SARS-CoV-2 through the postpandemic period.

Science. 2020-4-14

[8]
Estimates of the severity of coronavirus disease 2019: a model-based analysis.

Lancet Infect Dis. 2020-3-30

[9]
Virological assessment of hospitalized patients with COVID-2019.

Nature. 2020-4-1

[10]
Case-Fatality Rate and Characteristics of Patients Dying in Relation to COVID-19 in Italy.

JAMA. 2020-5-12

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